coa论文
1.ZHAO Guang-wu，ZHANG Guo-zhen and WANG Jian-hua．Seed Health Status Of Sweet Corn (Zea mays L. saccharata Sturt) From Five Areas In China And Effect On Field Seedling Emergence．Agricultural Sciences in China，2005，4（5）：329-335
2.Jiang Haiyan, Wang Jianhua, Wu Peng. 2005 Detection of genetically modified ingredients in seed samples from Heilongjiang soybean field. Chinese Science Bulletin, 2005, 50 (10): 975-979
3.Liu YJ, Song FP, HE KL, Yuan Y, Zhang XX, Gao P, Wang JH, Wang GY, 2004. Expression of a modified Cry1Ie gene in E. coli and in transgenic tobacco confers resistance to corn borer. Acta Biochimica et Biophysica Sinica. 36(4):309-313
4.Zhao HJ, Wang JH, Gao P, Gu RL, Zhang JQ, Wang TY, Wang GY, 2004.Cloning of plastid acetyl-CoA carboxylase cDNA from Setaria italica and sequence analysis of graminicide target site. Acta Botanica Sinica. 46(6):751-756
5.Yun-Jun LIU, Yuan YUAN, Jun ZHENG, Ya-Zhong TAO, Zhi-Gang DONG, Jian- Hua WANG, and Guo-Ying WANG, 2004. Signal Peptide of Potato PinII Enhances the Expression of Cry1Ac in Transgenic Tobacco. Acta Biochim Biophys Sin, 36(8): 553
『贰』 医学论文 摘要中译英
Abstract(摘要）:
There are various kinds of medicines of decreasing triglyceride and cholesterol of plasma.It is difficult to classify them because of their variety.According to the function,they usually are divided into four types:to decrease total cholesterol,mainly decrease cholesterol with secondary triglyceride,total triglyceride and mainly decrease triglyceride with secondary cholesterol.
Generally speaking,it can prevent cholic acid or cholesterol to be absorbed through enteric canal and promote them to be ejected by excrement.Besides,it can also restrain the internal combining of cholesterol,or accelerate its transformation,LDL receptor of periplasts and the decomposition of lipoprotein.And it can activate lipoprotein and metabolic enzymes,promote hydrolyzation of triglycercide,resist other lipids' combining or promote the metabolism.
At present,the antilipemic are mainly composed of the following: cholic acid chelating agent、HMG-CoA rectase inhibitor、niacin and its derivative、the Fibrates、Probucol、pantethine、elastase、Omega-3 fatty acid and others.
This paper indicates the antilipemic's current research development,including the following aspects:
Sums up the new research viewpoints and methods about the antilipemic and states the method of administration and curative mechanism of lovastatin、isoflavone、enteromorpha polysaccharide，etc.Then analyzes the concret solution and compares various medicines by ince blood fat's proction.With unceasing practice and comparison, we've proved that a great progress has been made in research of modern antilipemic and there are more effective treatment protocols anyway.
Key Words（关键词）：
antilipemic；triglycercide；lipoproteins；niacin；isoflavone
可完了~N多专业术语 呵呵 我也学了好多呢 祝论文顺利过关！
『叁』 生化论文翻译
Hfx. volcanii hmgA的基因,就像许多其它halobacterial基因,产生一个领袖,即没有5«领袖序列的开始共同上游。在一开始的转录后开始Lam&码(1992),第37约25元的基因上游典型的小AT-rich序列,archaeal发起人Danner强,1996年和Soppa(1995);帕和《;已等,1990;由Soppa,1999)。上游的Har.码的hispanica hmgA出现了类似的AT-rich序列在一个非常类似的距离为发起人,volcanii hmgA Hfx.(见图1)。与此一致的发起人,序列的抗药性的Har. hispanica基因显示一个基地替换(相比,在这AT-rich重基因),形成了一个序列接近一致强archaeal发起人。这种变化是相似的立场和类型的up-promoter突变的Hfx. volcanii hmgA(图1);Lam& 37,1992)。为了避免混乱与以往文献的基础上,Har.标记基因hispanica hmgA将作为Mevr轨迹,即便是他汀药物用于选择。Mevinolin和辛伐他汀非常相似,功能相当于化学,但Mevinolin不是商业销售在澳大利亚。
Har.建设hispanica hmgA-based飞船向量
『肆』 如何找到2012年COA的会议论文
好过，我COA重修过的，补考没学，重修认真看了一下，才发现coa如此简单，主要看大题，选择不给你原题基本很难选对，大题每年都是那几道，全答对就一半分了，选择和判断在蒙一下，就过了。软件学院12号楼的小书店有卖真题的，看看就过了
『伍』 何朝族的发表论文
1.Xiaoyu LI, Xiuxiu HAN, Zhiqiang LIU, Chaozu HE(2013). The function and properties of the transcriptional regulator COS1 in Magnaporthe oryzae. fungal biology 117:239-249（通讯作者）2.Daqing Mao, Jun Tao, Chunxia Li, Chao Luo, Linlin Zheng and Chaozu He (2012) Light signalling mediated by Per-ARNT-Sim domain-containing proteins in Xanthomonas campestris pv. campestris. FEMS Microbiology Letters 326:31-39（通讯作者）3Feng Feng Fan Yang, Wei Rong, Xiaogang Wu, She Chen, Chaozu He and Jian-Min Zhou (2012) A Xanthomonas uridine 5’-monophosphate transferase inhibits plant immune kinases. Nature 485: 114-118（通讯作者）4. Yan Zhang & Feng Feng and Chaozu He (2012) Downregulation of OsPK1 Contributes to Oxidative Stress and the Variations in ABA/GA Balance in Rice Plant Mol Biol Rep 30:1006–1013（通讯作者）5.Yan Zhang, Wenkai Xiao, ijuan Luo, inhuan Pang, ei Rong, Chaozu He（2012）. Downregulation of OsPK1, a cytosolic pyruvate kinase, by T-DNA insertion causes dwarfism and panicle enclosure in rice. Planta. 235:25-38（通讯作者）6.Daqing Mao,Jun Tao,Chunxia Li ,Chao Luo,Linlin Zheng,Chaozu He(2012) Identification of novel oxygen sensors using a combined approach in Xanthomonas campestris pv. Campestris. Ann Microbiol, 62:957–9647.Chunxia Li, Jun Tao, Daqing Mao, Chaozu He（2011）. A Novel Manganese Efflux System, YebN, Is Required for Virulence by Xanthomonas oryzae pv. oryzae. PLoS One 6: e21983. doi:10.1371/journal.pone.0021983（通讯作者）8.Daqing Mao, Jun Tao, Chunxia Li, Chao Luo, Linlin Zhengand Chaozu He(2011) A novel one-component system, XvgA involved in regulation of bacterial growth and virulence of Xanthomonas campestris pv. Campestris, 5(25): 4433-4441（通讯作者）9.Zhihui Xia, Huimin Xu , Jinling Zhai, Dejun Li, Hongli Luo, Chaozu He, Xi Huang（2011）. RNA-Seq analysis and de novo transcriptome assembly of Hevea brasiliensis. Plant Molecular Biology 77:299-308（通讯作者）10. Wei Rong, Feng Feng, Jian-Min Zhou and Chaozu He (2010) Effector-triggered innate immunity contributes Arabidopsis resistance to Xanthomonas campestris. Molecular Plant Pathology 11: 783-793. （通讯作者）11. Jun Tao and Chaozu He (2010) Response regulator,VemR, positively regulates thevirulence and adaptation of Xanthomonas campestris pv. campestris. FEMS Microbiology Letters 304: 20–28. （通讯作者）12. Zhuang Zhou, Guihua Li, Chunhua Lin and Chaozu He (2009) Conidiophore Stalk-less1 Encodes a Putative Zinc-Finger Protein Involved in the Early Stage of Conidiation and Mycelial Infection in Magnaporthe oryzae. Molecular Plant-Microbe Interactions 22: 402–410. （通讯作者）13. Wei Qian, Zhongji Han, Jun Tao and Chaozu He (2008) Genome-Scale Mutagenesis and Phenotypic Characterization of Two-Component Signal Transction Systems in Xanthomonas campestris pv. campestris ATCC 33913. Molecular Plant-Microbe Interactions 21:1128-1138. （通讯作者）14. Dongmei Yu, Kosala Ranathunge, Huasun Huang, Zhongyou Pei, Rochus Franke, Lukas Schreiber and Chaozu He (2008) Wax Crystal-Sparse Leaf1 encodes a β–ketoacyl CoA synthase involved in biosynthesis of cuticular waxes on rice leaf. Planta 228: 675–685. （通讯作者）15. Chao Ge and Chaozu He (2008) Regulation of the Type II secretion structural gene xpsE in Xanthomonas campestris pathovar campestris by the global transcription regulator Clp. Current Microbiology 56:122-127. （通讯作者）16. Lifeng Wang, Wei Rong and Chaozu He (2008) Two Xanthomonas extracellular polygalacturonases, PghAxc and PghBxc, are regulated by Type III secretion regulators HrpX and HrpG and are required for virulence. Molecular Plant-Microbe Interactions 21:555-563.（通讯作者）17. Wei Qian, Zhong-Ji Han and Chaozu He (2008) Two-Component Signal Transction Systems of Xanthomonas spp.: A Lesson from Genomics. Molecular Plant-Microbe Interactions 21:151-161.（通讯作者）18. Hong, W.-F.; He, C.-Z.; Wang, L.-J; Wang, D.-J.; Joseph, L. M.; Jantasuriyarat, C.; Dai, L.-Y. and Wang G.-L. (2007). BWMK1 Responds to Multiple Environmental Stresses and Plant Hormones. Journal of Integrative Plant Biology 49:843-85119. Hu, J.; Zhang, Y.; Qian, W. and Chaozu He (2007). Avirulence gene and insertion element based RFLP as well as RAPD markers reveal high levels of genomic polymorphism in the rice pathogen Xanthomonas oryzae pv. oryzae. Systematic and Applied Microbiology. 30:587–60020. Lifeng Wang, Xiaoyan Tang and Chaozu He (2007). The bifunctional effector AvrXccC of Xanthomonas campestris pv. campestris requires plasmamembrane-anchoring for host recognition. Molecular Plant Pathology 8: 491-501.（通讯作者）21. Chunxiao Xu and Chaozu He (2007). The rice OsLOL2 gene encodes a zinc finger protein involved in development and disease resistance. Molecular Genetics and Genomics 278: 85-94（通讯作者）。22. Jun Hu, Wei Qian and Chaozu He (2007). The Xanthomonas oryzae pv. oryzae eglXoB endoglucanase gene is required for virulence to rice. FEMS Microbiology Letters269: 273–279（通讯作者）。23. Guihua Li, Zhuang Zhou, Guifu Liu, Fucong Zheng and Chaozu He (2007) Characterization of T-DNA insertion patterns in the genome of rice blast fungus Magnaporthe oryzae. Current Genetics 51: 233-243（通讯作者）。24. Wei Qian, Yantao Jia, Shuang-Xi Ren, Yong-Qiang He Jia-Xun Feng, Ling-Feng Lu, Qihong Sun, Ge Ying, Dong-Jie Tang, Hua Tang, Wei Wu, Pei Hao, Lifeng Wang, Bo-Le Jiang, Shenyan Zeng, Wen-Yi Gu, Gang Lu, Li Rong, Yingchuan Tian, Zhijian Yao, Gang Fu, Baoshan Chen, Rongxiang Fang, Boqin Qiang, Zhu Chen, Guo-Ping Zhao, Ji-Liang Tang & Chaozu He (2005). Comparative and Functional Genomic Analyses for the Pathogenicity of Phytopathogen Xanthomonas campestris pv. campestris. Genome Research15: 757-767, （通讯作者）。25. Lijuan Wang, Zhongyou Pei, Yingchuan Tian and Chaozu He （2005）OsLSD1, a Rice Zinc Finger Protein, Regulates Programmed Cell Death and Callus Differentiation. Molecular Plant Microbe Interactions 18: 375-384（通讯作者）26. Guifu Liu, Lijuan Wang, Zhuang Zhou, Hei Leung, Guo-liang Wang and Chaozu He (2004). A rice lesion mimic gene, Spl1, is physically mapped and delimitated in a 70 kb segment of rice chromosome 12. Molecular Genetics and Genomics, 272: 108-115. （通讯作者）27. Qihong Sun, Jun Hu, Guixiu Huang, Chao Ge, Rongxiang Fang and Chaozu He (2005). The type II secretion pathway structural gene xpsE is required for xylanase- and cellulase- secretion and virulence in Xanthomonas oryzae pv. oryzae. Plant Pathology, 54: 15-21. （通讯作者）28. Lian-Hui Wang, Yawen He, Yunfeng Gao, Ji En Wu, Yi-Hu Dong, Chaozu He, Su Xing Wang, Li-Xing Weng, Jin-Ling Xu, Leng Tay, Rong Xiang Fang, Lian-Hui Zhang (2004). A bacterial cell-cell communication signal with cross-kingdom structural analogues. Molecular Microbiology 51:903-912.29. Youbao Sha, Shutian Li, Zhongyou Pei, Lijuan Luo, Yingchuan Tian and Chaozu He (2004). Generation and flanking sequence analysis of a rice T-DNA tagged population. Theoretical and Applied Genetics (TAG). 108: 306-314（通讯作者）30. Qihong Sun, Wei Wu, Wei Qian, Jun Hu, Rongxiang Fang and Chaozu He (2003). High-quality mutant libraries of Xanthomonas oryzae pv. oryzae and X. campestris pv. campestris generated by an efficient transposon mutagenesis system. FEMS Microbiology Letters 226:145-150. （通讯作者）31．Wang G.L., He C.Z., Wu C.J., Yin Z.C., Baraoidan M., Zeng L.R., Ronald P.C., Khush G.S. and Leung H. (2001) Genetic Dissection of Disease Resistance Pathways in Rice. Advances in Rice Blast Research. eds: M.H. Lebrun, J.L. Notteghem, N.J. Talbot and D. Tharreau. Kluwer Publisher, pp 63-72.32．Chaozu He, Steven H. T. Fong , Daichang Yang and Guo-Liang Wang (1999).BWMK1, a novel MAP kinase inced by fungal infection and mechanical wounding in rice. Molecular Plant-Microbe Interactions. 12:1064-1073.33．He, C., Rusu, A. G., Poplawski A. M., Irwin, J.A.G. and Manners, J.M. (1998). Transfer of a supernumery chromosome between vegetatively incompatible biotypes of the fungus Colletotrichum gloeosporioides. Genetics 150: 1459-1466.34．Manners, J.M., He, C. (1997) Molecular approaches to studies of Colletotrichum gloeosporioidescausing anthracnose of Stylosanthes in Australia. Tropical Grassland, 31: 435-444. (review paper)35．Poplawski A. M., He, C., Irwin, J.A.G. and Manners, J.M. (1997). Transfer of autonomously replicating vector between vegetatively incompatible biotypes of Colletotrichum gloeosporioides. Current Genetics, 32: 66-72.36．He, C., Nourse, J.P., Kelemu, S., Irwin, J.A.G. and Manners, J.M. (1996) CgT1: A non-LTR retrotransposon with restricted distribution in the fungal phytopathogen Colletotrichum gloeosporioides. Molecular and General Genetics. 252: 320-331.37．Masel AM*, He C.*, Poplawski AM, Irwin JAG, Manners JM (1996) Molecular evidence for chromosome transfer between biotypes of Colletotrichum gloeosporioides. Molecular Plant-Microbe Interactions, 9: 339-348 (*并列第一作者)38．He C, Masel AM, Irwin JAG, Kelemu S, Manners JM (1995). Relationship of chromosome-specific dispensable DNA sequences in diverse isolates Colletotrichum gloeosporioides. Mycological Research 99: 1325-1333.
『陆』 王伟的科研论文
1. 王伟，方学明，朱苹香.反相高效液相色谱法测定羟氨苄青霉素的血液浓度探讨.福建医药杂志，1998；20（4）：99-100获得上海市闵行区医学会论文评比三等奖
2. 王伟.微机在药房管理中的运用.中国药房，1999；10：18
3. 王伟，方学明，沈晓英.上海地区九十年代末用药分析.中国药房，2000；11：77-78 在“全国医院药学、临床用药论文评选活动”中，评为优秀论文。
4. 王伟.阿莫西林可溶片的药动学及生物利用度.中国医院药学杂志，2000；20（9）：87-88
5. 王伟.某院1999-2001年抗感染药物利用分析及对策.中国药师，2003；6（12）：827-829
6. 王伟，方学明.浅析我院的药品结构与促进合理用药措施.中国医院药学杂志，2003；23（9）：76-77
7. 王伟.药物引起荨麻疹1例.药物流行病学杂志，2003；12（6）：285
8. 《HMG-COA还原酶抑制剂的药理作用及临床应用》经专家评审在“98首届中国药师周-世纪之交的中国药学”大会进行交流。
9. 王伟，方学名.150份药历的用药合理性分析.中国药师，2002；5（4）：232-241
10. 王伟，沈晓英，曹华，等.地卡因胶浆的制备与应用.医药导报，2005；24：110
11. 王伟.高血压患者的药学监护.中国药师2004；7：12-13
『柒』 请帮忙找一篇基因工程相关的外文论文再加上翻译！
Synthesis of optically pure ethyl (S)-4-chloro-3-hydroxybutanoate
by Escherichia coli transformant cells coexpressing
the carbonyl rectase and glucose dehydrogenase genes
由共表达碳酰还原酶和葡萄糖脱氢酶的大肠杆菌转化细胞合成
纯光学（S）-4-氯-3-羟基丁酸乙酯
Abstract The asymmetric rection of ethyl 4-chloro-3-
oxobutanoate (COBE) to ethyl (S)-4-chloro-3-hydroxybutanoate
((S)-CHBE) was investigated. Escherichia coli cells expressing both the carbonyl rectase (S1) gene from Candida magnoliae and the glucose dehydrogenase (GDH) gene from Bacillus megaterium were used as the
catalyst. In an organic-solvent-water two-phase system,(S)-CHBE formed in the organic phase amounted to 2.58 M (430 g/l), the molar yield being 85%. E. coli transformant cells coprocing S1 and GDH accumulated 1.25 M (208 g/l) (S)-CHBE in an aqueous monophase system by continuously feeding on COBE, which is unstable in an aqueous solution. In this case, the calculated turnover of NADP+ (the oxidized form of nicotinamide adenine dinucleotide phosphate) to CHBE was 21,600 mol/mol. The optical purity of the (S)-CHBE formed was 100% enantiomeric excess in both systems. The aqueous system used for the rection reaction involving E. coli HB101 cells carrying a plasmid containing the S1 and GDH genes as a catalyst is simple. Furthermore, the system does not require the addition of commercially available GDH or an organic solvent. Therefore this system is highly advantageous for the practical synthesis of optically pure (S)-CHBE.
本本篇文献研究了利用COBE不对称合成（S）-4-氯-3-羟基丁酸乙酯（CHBE）。大肠杆菌细胞作为催化剂同时表达了来自念珠菌属magnoliae的碳酰还原酶和来自巨大芽孢杆菌的葡萄糖脱氢酶基因。在水/有机溶剂两相体系中，(S)-CHBE在有机相中的浓度可以达到2.58M（430g/l），摩尔产率达到85%。大肠杆菌的副产物S1和GDH也达到了1.25M（208g/l），COBE在水相中不稳定，所以(S)-CHBE可以在水单相中不停的生成。在这种情况下，适当的从NADP+到CHBE的转变达到了21,600 mol/mol。所形成的CHBE的旋光度在这种体系中100%对映体过量。在水相中用携带含有S1和GDH基因质粒的E. coli HB101作为催化剂不对称还原是比较简单的。并且，这种体系并不额外需要商业GDH或者有机溶剂。因此，这种体系对于实际合成纯光学活性的(S)-CHBE是非常方便的。
Optically active 4-chloro-3-hydroxybutanoic acid esters are useful chiral building blocks for the synthesis of pharmaceuticals. The (R)-enantiomer is a precursor of L-carnitine (Zhou et al. 1983), and (S)-enantiomer is an important starting material for hydroxymethylglutaryl- CoA (HMG-CoA) rectase inhibitors (Karanewsky et
al. 1990). Many studies have described the microbial or enzymatic asymmetric rection of 4-chloro-3-oxobutanoic acid esters (Aragozzini and Valenti 1992; Bare et al.1991; Hallinan et al. 1995; Patel et al. 1992; Shimizu et al. 1990; Wong et al. 1985) based on the rection by baker’s yeast (Zhou et al. 1983).We have previously showed that Candida magnoliae AKU4643 cells reced ethyl 4-chloro-3-oxobutanoate (COBE) to (S)-CHBE with an optical purity of 96% enantiomeric excess (e.e.) (Yasohara et al. 1999). As this yeast has at least three different stereoselective rectases (Wada et al. 1998, 1999a, b), the (S)-CHBE proced by this yeast was not optically pure. From among these three enzymes, an NADPH-dependent carbonyl rectase, designated as S1, was purified and characterized in some detail (Wada et al. 1998). We cloned and sequenced the gene encoding S1 and overexpressed it in Escherichia coli cells. This E. coli transformant reced COBE to optically pure (S)-CHBE in the presence of glucose, NADP+, and commercially available glucose dehydrogenase (GDH) as a cofactor generator (Yasohara
et al. 2000).
Here, we describe the construction of three E. coli transformants coexpressing the S1 from C. magnoliae and GDH from Bacillus megaterium genes and analyze the rection of COBE catalyzed by these strains. Previous reports on the enzymatic rection of COBE to (R)-CHBE with an optical purity of 92% e.e. (Kataoka et al. 1999; Shimizu et al. 1990) recommended an organic- solvent two-phase system reaction for an enzymatic or microbial rection, because the substrate (COBE) is unstable in an aqueous solvent and inactivates enzymes. We examined the rection of COBE to optically pure (S)-CHBE by E. coli transformants in a water monophase system reaction and discuss the possible use of this type of reaction system in instrial applications。
具有旋光性的（S）-4-氯-3-羟基丁酸乙酯在药物制剂的合成中是重要的手性化合物。其右旋体是L-卡尼汀的前体，其左旋体是羟甲基戊二酰辅酶A还原酶抑制剂的起始材料。许多研究描述了以面包酵母为基础微生物或者酶的COBE的不对称还原。我们先前已经知道利用来自念珠菌属magnoliae AKU4643 细胞催化COBE生成光学纯度96%的CHBE。这种酵母至少有三种立体选择性的还原酶，这种酵母产生的CHBE并非纯光学的，在这三种酶之中，NADPH-依赖碳酰还原酶，我们克隆并测序编码S1的基因，并在大肠杆菌中过表达。大肠杆菌转化细胞在葡萄糖，NADP+和商业化的葡萄糖脱氢酶作为辅酶因子的启动子催化COBE生成纯光学的CHBE。
我们构建这三种大肠杆菌转化细胞共表达来自的S1和来自巨大芽孢杆菌的GDH，并分析COBE被这几种菌株催化还原的反应机理。先前的报道表明，利用酶催化还原COBE生成CHBE光学纯度可达92%，也提到了因为底物（COBE）在水相中不稳定，并且酶容易钝化，所以利用酶或者微生物在有机溶剂/水两相体系中催化反应。我们研究了在水单相体系中由COBE还原生成纯光学的CHBE，还讨论了这种反应体系在工业应用中可能的用途。
Materials and methods
Bacterial strain and plasmids
The E. coli strains used in this study were JM109 and HB101.Plasmid pGDA2, in which the GDH gene from B. megaterium is inserted into pKK223-3, was kindly provided by Professor I. Urabe, Osaka University (Makino et al. 1989). Plasmids pSL301 and pTrc99A were purchased from Invitrogen (USA), and Amersham Pharmacia Biotech (UK), respectively. Plasmids pUC19 and pSTV28 (Homma et al. 1995; Takahashi et al. 1995) were purchased from Takara Shuzo (Japan).
材料和方法
菌株和质粒
本次实验中使用的大肠杆菌是JM109 and HB101。来自B. megaterium的GDH基因插入到Pkk233-3质粒中，而带有GDH基因片段的pGDA2质粒由到由大阪大学的urabe教授提供。质粒pSL301和 pTrc99A是由美国的Invitrogen公司和英国的公司分别购买的。质粒pUC19和pST28是由日本takara公司购买的。
The recombinant plasmid used in this study was constructed as follows (Fig. 1): Plasmid pGDA2 was double-digested with EcoRI and PstI to isolate a DNA fragment of about 0.9 kilobase pairs (kb) including the GDH gene. This fragment was inserted into the EcoRI-PstI site of plasmid pSL301 to construct plasmid pSLG. Plasmid pSLG was double-digested with EcoRI and XhoI to isolate a DNA fragment of about 0.9 kb including the GDH gene.
这次实验使用的重组质粒构建如下：质粒pGDA2 被EcoRI 和 PstI双酶切从而分离出一个大小约为0.9kb的包含有GDH基因的DNA片段。这个片段被插入到质粒Psl301的EcoRI-PstI酶切位点从而构建出质粒pSLG。质粒pSLG被EcoRI和XhoI
To construct plasmid pNTS1G, this 0.9-kb fragment was inserted into the EcoRI-SalI site of pNTS1, which was constructed to overproce S1 as described previously (Yasohara et al. 2000). To construct plasmid pNTGS1, plasmid pNTG was first generated. Two synthetic primers (primer 1, TAGTCCATATGTATAAAGATTTAG,and primer 2 TCTGAGAATTCTTATCCGCGTCCT) were prepared for polymerase chain reaction (PCR) using pGDA2 as the template. The PCR-generated fragment was double- digested with NdeI and EcoRI and then inserted into the NdeI EcoRI site of plasmid pUCNT, which was constructed from pUC19 and pTrc99A, as reported (Nanba et al. 1999), to obtain pNTG. To construct plasmid pNTGS1, two synthetic primers (primer 3, , and primer 4, ) were prepared using pUCHE, which contains the S1 gene as the template. The PCR-generated fragment was double-digested with EcoRI and SalI and then inserted into the EcoRI-SalI site of pNTG to obtain pNTGS1. Plasmid pNTS1G, pNTGS1 or pNTG was transformed into E. coli HB101.
构建pNTS1是为了过表达前文所提到的S1，这个0.9kb大小的片段被插入到pNTS1的EcoRI-SalI酶切位点从而构建pNTS1G。为了构建质粒pNTGS1，首先需要构建pNTG。两个合成引物（引物1，TAGTCCATATGTATAAAGATTTAG和引物2，TCTGAGAATTCTTATCCGCGTCCT）和作为模板的pGDA2是PCR反应需要的。PCR得到的片段是由NdeI 和EcoRI双酶切和并插入到质粒pUCNT的NdeI EcoRI酶切位点来得到pNTG。根据报道，pUCNT是由pUC19和 pTrc99A构建而来。为了构建质粒pNTGS1，两个合成引物(引物 3, , and 引物 4, )，包括了S1基因作为模板。Pcr产物片段被EcoRI和SalI双酶切然后被插入到pntg的EcoRI-SalI酶切位点得到pntg1.质粒pNTS1G, pNTGS1或者 pNTG都是导入大肠杆菌HB101.
Plasmid pGDA2 was double-digested with EcoRI and PstI to isolate a DNA fragment of about 0.9 kb including the GDH gene. To construct plasmid pSTVG, this fragment was inserted into the EcoRI-PstI site of plasmid pSTV28. Plasmid pSTVG was transformed into E. coli HB101.
质粒pGDA2被EcoRI 和 PstI双酶切得到包含GDH基因的0.9kb大小的DNA片段。为了构建pSTVG质粒，这个片段被插入到pSTV28质粒的EcoRI-PstI的酶切位点。pSTVG质粒被导入到E. coli HB101。
Medium and cultivation
The 2×YT medium comprised 1.6% Bacto-tryptone, 1.0% yeast
extract, and 0.5% NaCl, pH 7.0. E. coli HB 101 carrying pNTS1,
pNTG, pNTS1G, or pNTGS1 was inoculated into a test tube containing
2 ml 2×YT medium supplemented with 0.1 mg/ml ampicillin,
followed by incubation at 37 °C for 15 h with reciprocal shaking.
This preculture (0.5 ml) was transferred to a 500-ml shaking
flask containing 100 ml 2×YT medium. The cells were cultivated
at 37 °C for 13 h with reciprocal shaking. E. coli HB101 carrying
pNTS1 and pSTVG was similarly cultivated in 2×YT medium
supplemented with 0.1 mg/ml ampicillin and 0.1 mg/ml chloramphenicol.
培养基和培菌
2*YT培养基 包含有1.6%细菌用胰蛋白胨，1.0%酵母提取物，0.5% NaCl，pH7.0.
携带有pNTS1,pNTG, pNTS1G, 或 pNTGS1的大肠杆菌HB101被接种到有0.1mg/ml氨苄青霉素的2ml的2*YT培养基，37°C摇床15小时。将0.5ml菌液接种到100ml2*YT培养基的500ml烧瓶中。在37°C摇床培养13小时。携带有pNTS1 和 pSTVG质粒的大肠杆菌HB101在2*YT培养基中培养方法相似，只是培养基中要加入0.1 mg/ml的氨苄青霉素和 0.1 mg/ml的氯霉素。
Preparation of cell-free extracts and the enzyme assay
Cells were harvested from 100 ml of culture broth by centrifugation, suspended in 50 ml of 100 mM potassium phosphate buffer (pH 6.5), and then disrupted by ultrasonication. The cell debris was removed by centrifugation; the supernatant was recovered as the cell-free extract. Carbonyl rectase S1 activity (COBE-recing activity) was determined spectrophotometically as follows: The assay mixture consisted of 100 mM potassium phosphate buffer (pH 6.5), 0.1 mM NADPH, and 1 mM COBE. The reactions were incubated at 30 °C and monitored for the decrease in absorbance at 340 nm. The assay mixture for GDH activity consisted of 1 M Tris-HCl buffer (pH 8.0), 100 mM glucose, and 2 mM NADP+. The reactions were incubated at 25 °C and monitored for the increase in absorbance at 340 nm. One unit of S1 or GDH was defined as the amount catalyzing the rection of 1 μmol NADP+ or oxidation of 1 μmol NADPH per minute, respectively. Protein concentrations were measured with a protein
assay kit containing Coomassie brilliant blue (Nacalai Tesque, Japan),
using bovine serum albumin as the standard (Bradford 1976).
无细胞抽提液和酶鉴定
将100ml培养液离心收获菌体，用50ml0.1mol/LpH为6.5的磷酸缓冲液悬浮，然后超声粉碎。细胞碎片通过离心可以去除，收集上层清液就是无细胞抽提物。碳酰还原酶S1的活性由分光光度计测量如下：测定的混合物包括：0.1mol/LpH6.5的磷酸二氢钾缓冲液，0.1mMNADPH和1mMCOBE。反应在30°C条件下反应，并且随时监测其在340nm处的吸光值。测GDH混合物包括：1M pH 8.0的Tris-HCl的缓冲液，100mM的葡萄糖，2mM的NADP+。反应在25°C下进行，监测其在340nm处的吸光值。一个单位S1或GDH被定义为每分钟催化还原1μmol NADP+或氧化1 μmol NADPH的量。蛋白质的测定通过含有考马斯亮蓝的蛋白质测定试剂利用牛血清白蛋白作为标准进行测定。
Study of enzyme stability
One milliliter of 100 mM potassium phosphate buffer (pH 6.5) containing the cell-free extracts of E. coli HB101 carrying pNTS1 (S1: 20 U/ml) was mixed with an equal volume of each test organic solvent in a closed vessel. After the mixture was shaken at 30 °C for 48 h, the remaining enzyme activities in an aqueous phase were assayed as described above. The mixture, containing 100 mM potassium phosphate buffer (pH 6.5), S1 (20 U/ml), and various concentrations of CHBE, was incubated at 30 °C for 24 h in order to study the enzyme’s stability in the presence of CHBE.The remaining enzyme activities were assayed as described above.
酶稳定性的研究
一毫升含有含有pNTS1质粒的E. coli HB101的无细胞抽提液的100mM磷酸氢二钾缓冲液（pH6.5）与等体积的有机溶剂混合。混合物在30 °C震摇48小时后，水相中残留的酶活力即是上述的酶活力。
COBE rection with E. coli cells expressing the S1 gene and E. coli cells expressing GDH genes in a two-phase system reaction
The reaction mixture comprised 15 ml culture broth of E. coli HB101 carrying pNTG, 17 ml culture broth of E. coli HB101 carrying pNTS1, 1.6 mg NADP+, 4 g glucose, 2.5 g COBE, 25 ml n-butyl acetate, and about 25 mg Triton X-100. The pH of the reaction mixture was controlled at 6.5 with 5 M sodium hydroxide. At 2 h, 1.25 g COBE and 2.5 g glucose were added to the reaction mixture. To compare the reaction by E. coli transformant coexpressing the GDH and S1 genes, 30 ml culture broth of E. coli
HB101 carrying pNTS1G was used instead of culture broth of E. coli HB101 carrying pNTG and E. coli HB101 carrying pNTS1. Other components and the procere were the same as described above.
表达S1基因和GDH基因的大肠杆菌细胞在两相反应体系中的还原反应
混合物包含有带有pNTG质粒的大肠杆菌HB101的菌液15ml，pNTS1质粒的大肠杆菌HB101的菌液17ml，1.6 mg NADP+，4 g葡萄糖，2.5g的COBE，25ml的n-butyl acetate丁酰醋酸盐和大约25mg的聚乙二醇辛基苯基醚Triton X-100。用5M的NaOH溶液将pH控制在6.5。在反应两小时后，加入1.25gCOBE和2.5g葡萄糖到该混合物中。比较大肠杆菌转化细胞共表达GDH和S1基因，携带有pNTS1G质粒的大肠杆菌HB10130ml菌液取代了携带有pNTG和pNTS1质粒的大肠杆菌HB101菌液。其他的成分和步骤和上述的方法相似。
COBE rection to (S)-CHBE in a two-phase system reaction
The reaction mixture contained 50 ml of culture broth of an E. coli HB101 transformant, 3.2 mg NADP+, 11 g glucose, 10 g COBE, 50 ml n-butyl acetate, and about 50 mg Triton X-100. The reaction mixture was stirred at 30 °C, and the pH was controlled at 6.5 with 5 M sodium hydroxide. Five grams of COBE/5.5 g glucose and 10 g COBE/11 g glucose were added to the reaction mixture at 3 h and 7 h, respectively; 3.2 mg NADP+ was added at 26 h.
COBE在两相系统中还原生成（S）-CHBE
反应混合物包含50ml E. coli HB101转化细胞的培养液，3.2mgNADP+,11g
葡萄糖，10gCOBE，50ml丁酰醋酸，和大概50mg聚乙二醇辛基苯基醚Triton X-100.
在30°C温度下将其混合均匀，并用5M的NaOH溶液将pH控制在6.5。在第3小时加入5gCOBE和5.5g葡萄糖或者在第7小时加入10gCOBE和11g葡萄糖，分别在第26小时加入3.2gNADP+。
COBE rection to (S)-CHBE in an aqueous system reaction
The reaction mixture was made up of 50 ml of culture broth of an E. coli HB101 transformant, 3.1 mg NADP+, 11 g glucose, and about 50 mg Triton X-100. The reaction mixture was stirred at 30 °C. Fifteen grams of COBE was fed continuously by means of a micro-feeding machine at a rate of about 0.02 g/min for about 12 h. The pH of the reaction mixture was controlled at 6.5 with 5 M sodium hydroxide. The reaction mixture was extracted with 100 ml ethyl acetate. The organic layer was dried over anhydrous sodium sulfate and then evaporated in vacuo.
COBE在水相中还原成(S)-CHBE的反应
反应的体系是由50ml大肠杆菌HB101转化细胞的菌液，3.1mgNADP+，11g葡萄糖和大约50mg聚乙二醇辛基苯基醚Triton X-100。反应混合物在30°C15mg的COBE通过微量添加机器以0.02 g/min的速率连续12小时恒定的加入到体系中。用5M的NaOH溶液将pH控制在6.5。反应混合物用100ml乙酸乙酯萃取。有机层用无水硫酸钠吸干，并在真空中脱水。
Analysis
The organic layer was obtained on centrifugation of the reaction mixture and was assayed for CHBE and COBE by gas chromatography. Optical purity of CHBE was analyzed by high-performance liquid chromatography (HPLC), as described previously (Yasohara et al. 1999).
Enzymes and chemicals
Restriction enzymes and DNA polymerase were purchased from
Takara Shuzo (Japan). COBE (molecular weight: 164.59) was purchased
from Tokyo Kasei Kogyo (Japan). Racemic CHBE (molecular
weight: 166.60) was synthesized by rection of COBE with
NaBH4. All other chemicals used were of analytical grade and
commercially available.
分析
离心反应混合物得到的有机层通过气相色谱法测定其CHBE和COBE。COBE的光学纯度如前所述通过高效液相色谱法进行分析。
酶和化学试剂
限制性内切酶和DNA聚合酶由takara公司购得，COBE（分子量：164.59）由东京Tokyo Kasei Kogyo公司购得，消旋体CHBE（分子量166.6）通过COBE及NaBH4合成。所有其他化学试剂都是分析等级和商业化的试剂。
Construction of E. coli transformants overprocing S1 and GDH
To express the carbonyl rectase S1 and GDH genes in the same E. coli cells, four expression vectors were constructed (Fig. 1). Plasmids pNTS1G and pNTGS1 contain the S1 gene from C. magnoliae, the GDH gene from B. megaterium, the lac promoter derived from pUC19, and the terminator derived from pTrc99A. Plasmid pNTS1 contains the S1 gene, the lac promoter derived from pUC19, and the terminator derived from pTrc99A. The enzyme activities in cell-free extracts of the E. coli transformants are shown in Table 1. E. coli HB101 cells carrying the vector plasmid pUCNT had no detectable S1 or GDH activity. E. coli HB101 carrying either pNTS1G or pNTGS1 showed S1 and GDH activity without isopropyl-β-D-thiogalactopyranoside (IPTG) inction. The S1 activities of these two transformants were lower than the GDH activities. To obtain a transformant whose S1 activity was equal to or greater than the level of GDH activity, we used a lower vector, pSTV28 (Homma et al. 1995; Takahashi et al. 1995), to express the GDH gene. It may be possible to raise the S1 activity by lowering the GDH activity. Plasmid pSTVG contains the GDH gene, the lac promoter, the chloramphenicol resistance gene, and the replicative origin derived from pACYC184 for compatibility with the plasmid pNTS1. In E. coli HB101 carrying pNTS1 and pSTVG, the S1 activity was higher than the GDH activity, but this GDH
level may be too low to regenerate in a COBE rection reaction as described below.
过产生S1和GDH的大肠杆菌转化细胞的构建
为了在同一大肠杆菌细胞中表达碳酰还原酶S1和GDH基因，要构建四个表达型载体。质粒pNTS1G 和 pNTGS1包含有来自C. magnoliae的S1基因，来自B. megaterium的GDH基因，来自pUC19的LAC启动子，从pTrc99A的来的终止子，质粒pNTS1包含有S1基因，来自pUC19的LAC启动子，从pTrc99A的来的终止子。在大肠杆菌转化细胞的无细胞抽提物的酶活力如表一所示。携带有运输质粒pUCNT的大肠杆菌细胞无法检测到其S1和GDH活性。携带有pNTS1G 或 pNTGS1质粒在没有IPTG的诱导下有S1和GDH的活性。在这两个转化菌种中，S1的活力小于GDH的活力。为了得到S1活性等于或者大于GDH的大肠杆菌转化菌株，我们使用低拷贝的载体pSTV28，来表达GDH基因。它可能可以通过降低GDH的活性从而提高S1的活性。质粒pSTVG包含有GDH基因，lac启动子，和氯霉素抗性基因，以及与pNTS1具有相容性的从pACYC184得来的复制起始位点。在携带有pNTS1和pSTVG的大肠杆菌转化细胞中，S1的活性要高于GDH的活性，但是GDH的活性可能会太低而在COBE还原反应中不能再生。
太长了，字数有限制，所以不能发完。分数我无所谓啦，我很少登录的。这应该算是基因工程的吧，是我以前自己翻的，不是很好。如果你要的话可以联系我的邮箱。[email protected]
『捌』 黄路生的主要论著
发表论文160多篇，其中在20种国际性学术期刊发表SCI论文57篇；以通讯作者在17种国际性学术期刊发表SCI论文46篇，其中在本学科领域国际学术期刊排名前15% （Top 15%）的刊物发表论文21篇，有14篇发表于排名第1的刊物《Animal Genetics》，出版专（译）著教材2部，参编全国农林院校统编教材《动物遗传学》1部 。 权威核心刊物论文
1、Three novel quantitative trait loci for skin thickness in swine identified by linkage and genome-wide association studies；Huashui Ai, Shijun Xiao, Zhiyan Zhang, Bin Yang, Lin Li, Yuanmei Guo, Guoshan Lin, Jun Ren andLusheng Huang；Animal GeneticsVolume 45, Issue 4, pages 524–533, August 2014
2、Genetic Diversity, Linkage Disequilibrium and SelectionSignatures in Chinese and Western Pigs Revealed byGenome-Wide SNP Markers；Huashui Ai, Lusheng Huang, Jun Ren；PLoS ONE February 7, 2013
3、Fine mapping of a QTL for ear size on porcinechromosome 5 and identification of high mobilitygroup AT-hook 2 (HMGA2) as a positionalcandidate gene；Pinghua Li, Shijun Xiao, Na Wei, Zhiyan Zhang, Ruihua Huang, Yueqing Gu, Yuanmei Guo, Jun Ren,Lusheng Huang and Congying Chen；Genetics Selection Evolution 2012, 44:6
4、A comprehensive survey of number variation in 18 diverse pig populations and identification of candidate number variable genes associated with complex traits；Congying Chen, Lusheng Huang etc.；BMC Genomics 2012; 13: 733.
5、A global view of porcine transcriptome in threetissues from a full-sib pair with extremephenotypes in growth and fat deposition by paired-end RNA sequencing；Congying Chen,Huashui Ai, Jun Ren, Wanbo Li,Pinghua Li,Ruimin Qiao,Jing Ouyang, Ming Yang, Junwu Maand Lusheng Huang；BMC Genomics 2011, 12:448
6、The porcine MUC20 gene: molecular characterization and its association with susceptibility to enterotoxigenic Escherichia coli F4ab/ac；Ji H, Ren J, Yan X, Huang X, Zhang B, Zhang Z, Huang L.；Mol Biol Rep2011 Mar;38(3):1593-601
7、Adhesion phenotypes of pigs of Chinese and Western breeds and a White Duroc-Erhualian crossbreed with regard to susceptibility to enterotoxigenic Escherichia coli with fimbrial adhesins K99, 987P, and F41；Xueming Yan, Xiang Huang, Jun Ren, Jing Ouyang, MS Ming Yang, MS Pengfei Han, MS Lusheng Huang；American Journal of Veterinary ResearchJanuary 2011, Vol. 72, No. 1, Pages 80-84
8、Genetic variation within coat color genes of MC1R and ASIP in Chinese brownish red Tibetan pigs；MAO Huirong,REN Jun, DING Nengshui, XIAO Shijun, HUANG Lusheng；Animal Science JournalDecember 2010,630-634
9、A whole genome scanning for quantitative trait loci on traits related to sperm quality and ejaculation in pigs；Yuyun Xing, Jun Ren, Dongren Ren, Yuanmei Guo, Yanbo Wu, Guangcheng Yang, Huirong Mao, Bertram Brenig, Lusheng Huang；Animal Reproction ScienceAugust 2009, Pages 210–218
10、A genome-wide scan for quantitative trait loci affecting limb bone lengths and areal bone mineral density of the distal femur in a White Duroc × Erhualian F2 population；Huirong Mao, Yuanmei Guo, Guangcheng Yang, Bin Yang, Jun Ren, Sanfeng Liu, Huashui Ai, Junwu Ma, Bertram Brenig and Lusheng Huang；BMC Genetics 2008, 9:63
11、Quantitative trait loci for porcine baseline erythroid traits at three growth ages in a White Duroc � Erhualian F2 resource population；Zheng Zou; Jun Ren; Xueming Yan; Xiang Huang; Shujin Yang; Zhiyan Zhang; Bin Yang; Wanbo Li; Lusheng Huang；Mammalian Genome Sep2008, Vol. 19 Issue 9, p640
12、Isolation and molecular characterization of the porcine stearoyl-CoA desaturase gene；Ren J, Knorr C, Huang L, Brenig B；Gene2004 Sep 29;340(1):19-30.
13、Genetic variations of the porcine PRKAG3 gene in Chinese indigenous pig breeds；Lu-Sheng Huang,Jun-Wu Ma；Genetics Selection Evolution 2004, 36:481-486
重要核心刊物论文 作者题名来源黄路生找准问题 提升我国种猪育种水平《中国猪业》 2013年10期 黄路生种猪选育技术的现状与趋势《中国猪业》 2012年04期 任军 黄路生等仔猪断奶前腹泻抗病基因育种技术的创建及应用《猪业科学》2012年01期 肖石军 黄路生等采用全基因组扫描法定位影响猪后腿质量QTL《畜牧兽医学报》2009年10期 刘三凤 黄路生等鸡SCD和Sirt1基因的SNP搜寻及其遗传多样性分析《畜牧兽医学报》 2008年03期 晏学明 黄路生等不同品种猪α1-岩藻糖转移酶基因遗传变异初析《中国畜牧杂志》 2004年03期 邓素华 黄路生等猪黑素皮质素受体1（MC1R）基因与毛色表型的研究《遗传学报》2003年10期 陈从英 黄路生等牛早期胚胎性别鉴定PCR反应体系的优化研究《畜牧兽医学报》 2003年03期 任军 黄路生 等24个中外猪种（群）的AFLP多态性及其群体遗传关系《遗传学报》2002年09期 陈克飞 黄路生猪雌激素受体（ESR）基因对产仔数性状的影响《遗传学报》 2000年10期 黄路生 罗明等中俄猪16个遗传标记基因位点的多态与繁殖性状的相关研究《畜牧兽医学报》1999年05期 丁能水 黄路生等氟烷基因对猪繁殖性能影响的研究《江西农业大学学报》1999年04期 林树茂 黄路生等中国地方猪种TfC的基因频率与繁殖性能相关性分析《江西畜牧兽医杂志》1996年02期 黄路生遗传标记——现代猪育种工作的一项生物新技术《全球科技经济瞭望》1993年03期 书名出版社时间类型动物遗传学中国农业出版社2003参编 江西农业大学校史（二）江西高校出版社2010合著
『玖』 宋保亮的发表文章
Representative Publications:
1) Chu BB, Liao YC, Qi W, Xie C, Du X, Wang J, Yang H, Miao HH, Li BL and Song BL*. Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts. Cell, 161(2):291-306, 2015
2) Li PS, Fu ZY, Zhang YY, Xu CQ, Ma YT, Li BL and Song BL*. The clathrin adaptor Numb regulates intestinal cholesterol absorption through dynamic interaction with NPC1L1. Nature Medicine, 20(1): 80-86, 2014
3) Liu TF, Tang JJ, Li PS, Shen Y, Li JG, Miao HH, Li BL* and Song BL*. Ablation of gp78 in liver improves hyperlipidemia and insulin resistance by inhibiting SREBP to decrease lipid biosynthesis. Cell Metabolism, 16: 213-225, 2012
4) Tang JJ, Li JG, Qi W, Qiu WW, Li PS, Li BL and Song BL*. Inhibition of SREBP by a small molecule, betulin, improves hyperlipidemia and insulin resistance and reces atherosclerotic plaques. Cell Metabolism, 13: 44-56, 2011
5) Ge L, Wang J, Qi W, Miao HH, Cao J, Qu YX, Li BL and Song BL*. The cholesterol absorption inhibitor ezetimibe acts by blocking the sterol-inced internalization of NPC1L1. Cell Metabolism,7: 508-519, 2008
6) Cao J, Wang J, Qi W, Miao HH, DeBose-Boyd RA, Wang J, Li BL* and Song BL*. Ufd1 is a cofactor of gp78 and plays a key role in cholesterol metabolism. Cell Metabolism, 6:115-128, 2007
7) Ge L, Qi W, Wang LJ, Miao HH, Qu YX, Li BL and Song BL*. Flotillins play an essential role in Niemann-Pick C1 Like 1-mediated cholesterol uptake. PNAS, 108(2): 551-6, 2011
8) Song BL, Sever N, and DeBose-Boyd RA. Gp78, a membrane anchored ubiquitin ligase, associates with Insig-1 and couples sterol-regulated ubiquitination to degradation of HMG CoA rectase. Molecular Cell. 19(6):829-840, 2005
9) Song BL, Javitt NB, and DeBose-Boyd RA. Insig-mediated degradation of HMG CoA rectase stimulated by lanosterol, an intermediate in the synthesis of cholesterol. Cell Metabolism, 1: 179-189, 2005
10) Sever N#, Song BL#, Yabe D#, Goldstein JL, Brown MS, and DeBose-Boyd RA. Insig-dependent ubiquitination and degradation of mammalian 3-Hydroxy-3-methylglutaryl-CoA rectase stimulated by sterols and geranylgeraniol. J Biol Chem, 278: 52479-52490, 2003
Other Publications:
11) Wei J, Fu ZY, Li PS, Miao HH, Li BL, Ma YT, Song BL*. The Clathrin Adaptor Proteins ARH, Dab2, and Numb Play Distinct Roles in Niemann-Pick C1-Like 1 Versus Low Density Lipoprotein Receptor-mediated Cholesterol Uptake. J Biol Chem, 289(48):33689-700, 2014
12) Jiang W, Tang JJ, Miao HH, Qu YX, Qin J, Xu J, Yang J, Li BL, Song BL*. Forward Genetic Screening for Regulators Involved in Cholesterol Synthesis Using Validation-Based Insertional Mutagenesis. PLoS One, 9(11):e112632, 2014
13) Jiang W, Song BL*. Ubiquitin ligases in cholesterol metabolism. Diabetes Metab J. 38(3):171-80, 2014
14) Rogers MA, Liu J, Song BL, Li BL, Chang CC, Chang TY. Acyl-CoA: cholesterol acyltransferases (ACATs/SOATs): Enzymes with multiple sterols as substrates and as activators. J Steroid Biochem Mol Biol. S0960-0760(14): 00207-6, 2014
15) Xiao X*, Song BL. SREBP: a novel therapeutic target.Acta Biochim Biophys Sin (Shanghai).45(1):2-10, 2013
16) Song BL*. A special issue on 'Metabolism'. Acta Biochim Biophys Sin (Shanghai).45(1):1, 2013
17) Xu J, Hu G, Lu M, Xiong Y, Li Q, Chang CC, Song BL, Chang TY, Li BL. MiR-9 reces human acyl-coenzyme A:cholesterol acyltransferase-1 to decrease THP-1 macrophage-derived foam cell formation.Acta Biochim Biophys Sin (Shanghai).45(11):953-62, 2013
18) Lu M, Hu XH, Li Q, Xiong Y, Hu GJ, Xu JJ, Zhao XN, Wei XX, Chang CC, Liu YK, Nan FJ, Li J, Chang TY, Song BL*, Li BL*.A specific cholesterol metabolic pathway is established in a subset of HCCs for tumor growth.J Mol Cell Biol. 5:404-15,2013
19) Hu GJ, Chen J, Zhao XN, Xu JJ, Guo DQ, Lu M, Zhu M, Xiong Y, Li Q, Chang CC, Song BL, Chang TY, Li BL. Proction of ACAT1 56-kDa isoform in human cells via trans-splicing involving the ampicillin resistance gene.Cell Res. 23(8):1007-24, 2013
20) Liu Y, Xu XH, Chen Q, Wang T, Deng CY, Song BL, Du JL, Luo ZG. Myosin Vb controls biogenesis of post-Golgi Rab10 carriers ring axon development.Nat Commun. 4:2005.2013
21) Xie C, Zhou ZS, Li N, Bian Y, Wang YJ, Wang LJ, Li BL* and Song BL*. Ezetimibe blocks the internalization of NPC1L1 and cholesterol in mouse small intestine. J Lipid Res, 53: 2092-2101, 2012
22) Wang LJ and Song BL*. Niemann-Pick C1-Like 1 and cholesterol uptake. Biochim Biophys Acta, 1821(7):964-72, 2012
23) Xie C, Li N, Chen ZJ, Li BL, Song BL*. The small GTPase Cdc42 interacts with Niemann-Pick C1 Like 1 (NPC1L1) and controls its movement from endocytic recycling compartment to plasma membrane in a cholesterol dependent manner.J Biol Chem, 286(41):35933-42, 2011
24) Zhang JH, Ge L, Qi W, Zhang L, Miao HH, Li BL, Yang M and Song BL*. The N-terminal domain of NPC1L1 protein binds cholesterol and plays essential roles in cholesterol uptake. J Biol Chem, 286(28): 25088-97, 2011
25) Wang LJ, Wang J, Li N, Ge L, Li BL and Song BL*. Molecular characterization of the NPC1L1 variants identified from cholesterol low absorbers. J Biol Chem, 286(9): 7397-7408, 2011
26) Miao HH, Jiang W, Ge L, Li BL, Song BL*. Tetra-glutamic acid resies adjacent to Lys248 in HMG-CoA rectase are critical for the ubiquitination mediated by gp78 and UBE2G2. Acta Biochim Biophys Sin, 42(5): 303-310, 2010
27) Chu BB, Ge L, Xie C, Zhao Y, Miao HH, Wang J, Li BL and Song BL*. Requirement of Myosin Vb/Rab11a/Rab11-FIP2 complex in cholesterol-regulatedtranslocation of Niemann-Pick C1 Like 1 protein to the cell surface. J Biol Chem, 284: 22481-90, 2009.
28) Wang J, Chu BB, Ge L, Li BL, Yan Y* and Song BL*. Membrane topology of human NPC1L1, a key protein in enterohepatic cholesterol absorption. J Lipid Res, 50: -62, 2009
29) Lei L, Xiong Y, Chen J, Yang JB, Wang Y, Yang XY, Chang CCY, Song BL, Chang TY and Li BL*. TNF-alpha stimulates the ACAT1 expressionin differentiating monocytes to promote the CE-laden cell formation. J Lipid Res, 50: 1057-67, 2009
30) Zhao XN, Chen J, Lei L, Hu GJ, Xiong Y, Xu JJ, Li Q, Yang XY, Chang C, Song BL, Chang TY and Li BL. The optional long 5'-untranslated region of human ACAT1 mRNAs impairs the proction of ACAT1 protein by promoting its mRNA decay. Acta Biochim Biophys Sin, 41: 30-41, 2009
31) Chen J, Zhao XN, Yang L, Hu GJ, Lu M, Xiong Y, Yang XY, Chang CC, Song BL, Chang TY, Li BL. RNA secondary structures located in the interchromosomal region of human ACAT1 chimeric mRNA are required to proce the 56-kDa isoform. Cell Res, 18: 921-936, 2008
32) Qi W and Song BL*. Dissecting the NPC1L1-mediated cholesterol absorption. Future Lipidology, 3: 481-484, 2008
33) Cao J, Qi W and Song BL*. Tocotrienols and the regulation of cholesterol biosynthesis. Chapter 18 (p237-256) In:Tocotrienols: Beyond Vitamin E,CRC press, 2008
34) Lee JN, Song BL, DeBose-Boyd RA, Ye J. Sterol-regulated degradation of Insig-1 mediated by the membrane-bound ubiquitin ligase gp78. J Biol Chem, 281:39308-39315, 2006
35) Song BL* and Debose-Boyd RA*. Insig-dependent ubiquitination and degradation of 3-Hydroxy-3-methylglutaryl-Coenzyme A stimulated by {delta}- and {gamma}-Tocotrienols. J Biol Chem, 281: 25054-25061, 2006
36) Song BL, Wang CH, Yao XM, Yang L, Zhang WJ, Wang ZZ, Zhao XN, Yang JB, Qi W, Yang XY, Inoue K, Lin ZX, Zhang HZ, Kodama T, Chang CC, Liu YK, Chang TY and Li BL. Human acyl-CoA:cholesterolacyltransferase 2 gene expression in intestinal Caco-2 cells and in hepatocellular carcinoma. Bio chem J, 394: 617-626, 2006
37) Yao XM, Wang CH, Song BL, Yang XY, Wang ZZ, Qi W, Lin ZX, Chang CC, Chang TY andLi BL. Two human ACAT2 mRNA variants proced by alternative splicing and coding for novel isoenzymes. Acta Biochim Biophys Sin, 37: 797-806, 2005
38) Sever N, Lee PCW, Song BL, Rawson RB, and DeBose-Boyd RA. Isolation of mutant cells lacking Insig-1 through selection with SR-12813, an agent that stimulates degradation of 3-Hydroxy-3-methylglutaryl-Coenzyme A rectase. J Biol Chem, 279: 43136-43147, 2004
39) Song BL and Debose-Boyd RA. Ubiquitination of 3-Hydroxy-3-methylglutaryl-CoA rectase in permeabilized cells mediated by cytosolic E1 and a putative membrane-bound ubiquitin ligase. J Biol Chem, 279: 28798-28806, 2004