coa論文
1.ZHAO Guang-wu，ZHANG Guo-zhen and WANG Jian-hua．Seed Health Status Of Sweet Corn (Zea mays L. saccharata Sturt) From Five Areas In China And Effect On Field Seedling Emergence．Agricultural Sciences in China，2005，4（5）：329-335
2.Jiang Haiyan, Wang Jianhua, Wu Peng. 2005 Detection of genetically modified ingredients in seed samples from Heilongjiang soybean field. Chinese Science Bulletin, 2005, 50 (10): 975-979
3.Liu YJ, Song FP, HE KL, Yuan Y, Zhang XX, Gao P, Wang JH, Wang GY, 2004. Expression of a modified Cry1Ie gene in E. coli and in transgenic tobacco confers resistance to corn borer. Acta Biochimica et Biophysica Sinica. 36(4):309-313
4.Zhao HJ, Wang JH, Gao P, Gu RL, Zhang JQ, Wang TY, Wang GY, 2004.Cloning of plastid acetyl-CoA carboxylase cDNA from Setaria italica and sequence analysis of graminicide target site. Acta Botanica Sinica. 46(6):751-756
5.Yun-Jun LIU, Yuan YUAN, Jun ZHENG, Ya-Zhong TAO, Zhi-Gang DONG, Jian- Hua WANG, and Guo-Ying WANG, 2004. Signal Peptide of Potato PinII Enhances the Expression of Cry1Ac in Transgenic Tobacco. Acta Biochim Biophys Sin, 36(8): 553
『貳』 醫學論文 摘要中譯英
Abstract(摘要）:
There are various kinds of medicines of decreasing triglyceride and cholesterol of plasma.It is difficult to classify them because of their variety.According to the function,they usually are divided into four types:to decrease total cholesterol,mainly decrease cholesterol with secondary triglyceride,total triglyceride and mainly decrease triglyceride with secondary cholesterol.
Generally speaking,it can prevent cholic acid or cholesterol to be absorbed through enteric canal and promote them to be ejected by excrement.Besides,it can also restrain the internal combining of cholesterol,or accelerate its transformation,LDL receptor of periplasts and the decomposition of lipoprotein.And it can activate lipoprotein and metabolic enzymes,promote hydrolyzation of triglycercide,resist other lipids' combining or promote the metabolism.
At present,the antilipemic are mainly composed of the following: cholic acid chelating agent、HMG-CoA rectase inhibitor、niacin and its derivative、the Fibrates、Probucol、pantethine、elastase、Omega-3 fatty acid and others.
This paper indicates the antilipemic's current research development,including the following aspects:
Sums up the new research viewpoints and methods about the antilipemic and states the method of administration and curative mechanism of lovastatin、isoflavone、enteromorpha polysaccharide，etc.Then analyzes the concret solution and compares various medicines by ince blood fat's proction.With unceasing practice and comparison, we've proved that a great progress has been made in research of modern antilipemic and there are more effective treatment protocols anyway.
Key Words（關鍵詞）：
antilipemic；triglycercide；lipoproteins；niacin；isoflavone
可完了~N多專業術語 呵呵 我也學了好多呢 祝論文順利過關！
『叄』 生化論文翻譯
Hfx. volcanii hmgA的基因,就像許多其它halobacterial基因,產生一個領袖,即沒有5«領袖序列的開始共同上游。在一開始的轉錄後開始Lam&碼(1992),第37約25元的基因上游典型的小AT-rich序列,archaeal發起人Danner強,1996年和Soppa(1995);帕和《;已等,1990;由Soppa,1999)。上游的Har.碼的hispanica hmgA出現了類似的AT-rich序列在一個非常類似的距離為發起人,volcanii hmgA Hfx.(見圖1)。與此一致的發起人,序列的抗葯性的Har. hispanica基因顯示一個基地替換(相比,在這AT-rich重基因),形成了一個序列接近一致強archaeal發起人。這種變化是相似的立場和類型的up-promoter突變的Hfx. volcanii hmgA(圖1);Lam& 37,1992)。為了避免混亂與以往文獻的基礎上,Har.標記基因hispanica hmgA將作為Mevr軌跡,即便是他汀葯物用於選擇。Mevinolin和辛伐他汀非常相似,功能相當於化學,但Mevinolin不是商業銷售在澳大利亞。
Har.建設hispanica hmgA-based飛船向量
『肆』 如何找到2012年COA的會議論文
好過，我COA重修過的，補考沒學，重修認真看了一下，才發現coa如此簡單，主要看大題，選擇不給你原題基本很難選對，大題每年都是那幾道，全答對就一半分了，選擇和判斷在蒙一下，就過了。軟體學院12號樓的小書店有賣真題的，看看就過了
『伍』 何朝族的發表論文
1.Xiaoyu LI, Xiuxiu HAN, Zhiqiang LIU, Chaozu HE(2013). The function and properties of the transcriptional regulator COS1 in Magnaporthe oryzae. fungal biology 117:239-249（通訊作者）2.Daqing Mao, Jun Tao, Chunxia Li, Chao Luo, Linlin Zheng and Chaozu He (2012) Light signalling mediated by Per-ARNT-Sim domain-containing proteins in Xanthomonas campestris pv. campestris. FEMS Microbiology Letters 326:31-39（通訊作者）3Feng Feng Fan Yang, Wei Rong, Xiaogang Wu, She Chen, Chaozu He and Jian-Min Zhou (2012) A Xanthomonas uridine 5』-monophosphate transferase inhibits plant immune kinases. Nature 485: 114-118（通訊作者）4. Yan Zhang & Feng Feng and Chaozu He (2012) Downregulation of OsPK1 Contributes to Oxidative Stress and the Variations in ABA/GA Balance in Rice Plant Mol Biol Rep 30:1006–1013（通訊作者）5.Yan Zhang, Wenkai Xiao, ijuan Luo, inhuan Pang, ei Rong, Chaozu He（2012）. Downregulation of OsPK1, a cytosolic pyruvate kinase, by T-DNA insertion causes dwarfism and panicle enclosure in rice. Planta. 235:25-38（通訊作者）6.Daqing Mao,Jun Tao,Chunxia Li ,Chao Luo,Linlin Zheng,Chaozu He(2012) Identification of novel oxygen sensors using a combined approach in Xanthomonas campestris pv. Campestris. Ann Microbiol, 62:957–9647.Chunxia Li, Jun Tao, Daqing Mao, Chaozu He（2011）. A Novel Manganese Efflux System, YebN, Is Required for Virulence by Xanthomonas oryzae pv. oryzae. PLoS One 6: e21983. doi:10.1371/journal.pone.0021983（通訊作者）8.Daqing Mao, Jun Tao, Chunxia Li, Chao Luo, Linlin Zhengand Chaozu He(2011) A novel one-component system, XvgA involved in regulation of bacterial growth and virulence of Xanthomonas campestris pv. Campestris, 5(25): 4433-4441（通訊作者）9.Zhihui Xia, Huimin Xu , Jinling Zhai, Dejun Li, Hongli Luo, Chaozu He, Xi Huang（2011）. RNA-Seq analysis and de novo transcriptome assembly of Hevea brasiliensis. Plant Molecular Biology 77:299-308（通訊作者）10. Wei Rong, Feng Feng, Jian-Min Zhou and Chaozu He (2010) Effector-triggered innate immunity contributes Arabidopsis resistance to Xanthomonas campestris. Molecular Plant Pathology 11: 783-793. （通訊作者）11. Jun Tao and Chaozu He (2010) Response regulator,VemR, positively regulates thevirulence and adaptation of Xanthomonas campestris pv. campestris. FEMS Microbiology Letters 304: 20–28. （通訊作者）12. Zhuang Zhou, Guihua Li, Chunhua Lin and Chaozu He (2009) Conidiophore Stalk-less1 Encodes a Putative Zinc-Finger Protein Involved in the Early Stage of Conidiation and Mycelial Infection in Magnaporthe oryzae. Molecular Plant-Microbe Interactions 22: 402–410. （通訊作者）13. Wei Qian, Zhongji Han, Jun Tao and Chaozu He (2008) Genome-Scale Mutagenesis and Phenotypic Characterization of Two-Component Signal Transction Systems in Xanthomonas campestris pv. campestris ATCC 33913. Molecular Plant-Microbe Interactions 21:1128-1138. （通訊作者）14. Dongmei Yu, Kosala Ranathunge, Huasun Huang, Zhongyou Pei, Rochus Franke, Lukas Schreiber and Chaozu He (2008) Wax Crystal-Sparse Leaf1 encodes a β–ketoacyl CoA synthase involved in biosynthesis of cuticular waxes on rice leaf. Planta 228: 675–685. （通訊作者）15. Chao Ge and Chaozu He (2008) Regulation of the Type II secretion structural gene xpsE in Xanthomonas campestris pathovar campestris by the global transcription regulator Clp. Current Microbiology 56:122-127. （通訊作者）16. Lifeng Wang, Wei Rong and Chaozu He (2008) Two Xanthomonas extracellular polygalacturonases, PghAxc and PghBxc, are regulated by Type III secretion regulators HrpX and HrpG and are required for virulence. Molecular Plant-Microbe Interactions 21:555-563.（通訊作者）17. Wei Qian, Zhong-Ji Han and Chaozu He (2008) Two-Component Signal Transction Systems of Xanthomonas spp.: A Lesson from Genomics. Molecular Plant-Microbe Interactions 21:151-161.（通訊作者）18. Hong, W.-F.; He, C.-Z.; Wang, L.-J; Wang, D.-J.; Joseph, L. M.; Jantasuriyarat, C.; Dai, L.-Y. and Wang G.-L. (2007). BWMK1 Responds to Multiple Environmental Stresses and Plant Hormones. Journal of Integrative Plant Biology 49:843-85119. Hu, J.; Zhang, Y.; Qian, W. and Chaozu He (2007). Avirulence gene and insertion element based RFLP as well as RAPD markers reveal high levels of genomic polymorphism in the rice pathogen Xanthomonas oryzae pv. oryzae. Systematic and Applied Microbiology. 30:587–60020. Lifeng Wang, Xiaoyan Tang and Chaozu He (2007). The bifunctional effector AvrXccC of Xanthomonas campestris pv. campestris requires plasmamembrane-anchoring for host recognition. Molecular Plant Pathology 8: 491-501.（通訊作者）21. Chunxiao Xu and Chaozu He (2007). The rice OsLOL2 gene encodes a zinc finger protein involved in development and disease resistance. Molecular Genetics and Genomics 278: 85-94（通訊作者）。22. Jun Hu, Wei Qian and Chaozu He (2007). The Xanthomonas oryzae pv. oryzae eglXoB endoglucanase gene is required for virulence to rice. FEMS Microbiology Letters269: 273–279（通訊作者）。23. Guihua Li, Zhuang Zhou, Guifu Liu, Fucong Zheng and Chaozu He (2007) Characterization of T-DNA insertion patterns in the genome of rice blast fungus Magnaporthe oryzae. Current Genetics 51: 233-243（通訊作者）。24. Wei Qian, Yantao Jia, Shuang-Xi Ren, Yong-Qiang He Jia-Xun Feng, Ling-Feng Lu, Qihong Sun, Ge Ying, Dong-Jie Tang, Hua Tang, Wei Wu, Pei Hao, Lifeng Wang, Bo-Le Jiang, Shenyan Zeng, Wen-Yi Gu, Gang Lu, Li Rong, Yingchuan Tian, Zhijian Yao, Gang Fu, Baoshan Chen, Rongxiang Fang, Boqin Qiang, Zhu Chen, Guo-Ping Zhao, Ji-Liang Tang & Chaozu He (2005). Comparative and Functional Genomic Analyses for the Pathogenicity of Phytopathogen Xanthomonas campestris pv. campestris. Genome Research15: 757-767, （通訊作者）。25. Lijuan Wang, Zhongyou Pei, Yingchuan Tian and Chaozu He （2005）OsLSD1, a Rice Zinc Finger Protein, Regulates Programmed Cell Death and Callus Differentiation. Molecular Plant Microbe Interactions 18: 375-384（通訊作者）26. Guifu Liu, Lijuan Wang, Zhuang Zhou, Hei Leung, Guo-liang Wang and Chaozu He (2004). A rice lesion mimic gene, Spl1, is physically mapped and delimitated in a 70 kb segment of rice chromosome 12. Molecular Genetics and Genomics, 272: 108-115. （通訊作者）27. Qihong Sun, Jun Hu, Guixiu Huang, Chao Ge, Rongxiang Fang and Chaozu He (2005). The type II secretion pathway structural gene xpsE is required for xylanase- and cellulase- secretion and virulence in Xanthomonas oryzae pv. oryzae. Plant Pathology, 54: 15-21. （通訊作者）28. Lian-Hui Wang, Yawen He, Yunfeng Gao, Ji En Wu, Yi-Hu Dong, Chaozu He, Su Xing Wang, Li-Xing Weng, Jin-Ling Xu, Leng Tay, Rong Xiang Fang, Lian-Hui Zhang (2004). A bacterial cell-cell communication signal with cross-kingdom structural analogues. Molecular Microbiology 51:903-912.29. Youbao Sha, Shutian Li, Zhongyou Pei, Lijuan Luo, Yingchuan Tian and Chaozu He (2004). Generation and flanking sequence analysis of a rice T-DNA tagged population. Theoretical and Applied Genetics (TAG). 108: 306-314（通訊作者）30. Qihong Sun, Wei Wu, Wei Qian, Jun Hu, Rongxiang Fang and Chaozu He (2003). High-quality mutant libraries of Xanthomonas oryzae pv. oryzae and X. campestris pv. campestris generated by an efficient transposon mutagenesis system. FEMS Microbiology Letters 226:145-150. （通訊作者）31．Wang G.L., He C.Z., Wu C.J., Yin Z.C., Baraoidan M., Zeng L.R., Ronald P.C., Khush G.S. and Leung H. (2001) Genetic Dissection of Disease Resistance Pathways in Rice. Advances in Rice Blast Research. eds: M.H. Lebrun, J.L. Notteghem, N.J. Talbot and D. Tharreau. Kluwer Publisher, pp 63-72.32．Chaozu He, Steven H. T. Fong , Daichang Yang and Guo-Liang Wang (1999).BWMK1, a novel MAP kinase inced by fungal infection and mechanical wounding in rice. Molecular Plant-Microbe Interactions. 12:1064-1073.33．He, C., Rusu, A. G., Poplawski A. M., Irwin, J.A.G. and Manners, J.M. (1998). Transfer of a supernumery chromosome between vegetatively incompatible biotypes of the fungus Colletotrichum gloeosporioides. Genetics 150: 1459-1466.34．Manners, J.M., He, C. (1997) Molecular approaches to studies of Colletotrichum gloeosporioidescausing anthracnose of Stylosanthes in Australia. Tropical Grassland, 31: 435-444. (review paper)35．Poplawski A. M., He, C., Irwin, J.A.G. and Manners, J.M. (1997). Transfer of autonomously replicating vector between vegetatively incompatible biotypes of Colletotrichum gloeosporioides. Current Genetics, 32: 66-72.36．He, C., Nourse, J.P., Kelemu, S., Irwin, J.A.G. and Manners, J.M. (1996) CgT1: A non-LTR retrotransposon with restricted distribution in the fungal phytopathogen Colletotrichum gloeosporioides. Molecular and General Genetics. 252: 320-331.37．Masel AM*, He C.*, Poplawski AM, Irwin JAG, Manners JM (1996) Molecular evidence for chromosome transfer between biotypes of Colletotrichum gloeosporioides. Molecular Plant-Microbe Interactions, 9: 339-348 (*並列第一作者)38．He C, Masel AM, Irwin JAG, Kelemu S, Manners JM (1995). Relationship of chromosome-specific dispensable DNA sequences in diverse isolates Colletotrichum gloeosporioides. Mycological Research 99: 1325-1333.
『陸』 王偉的科研論文
1. 王偉，方學明，朱蘋香.反相高效液相色譜法測定羥氨苄青黴素的血液濃度探討.福建醫葯雜志，1998；20（4）：99-100獲得上海市閔行區醫學會論文評比三等獎
2. 王偉.微機在葯房管理中的運用.中國葯房，1999；10：18
3. 王偉，方學明，沈曉英.上海地區九十年代末用葯分析.中國葯房，2000；11：77-78 在「全國醫院葯學、臨床用葯論文評選活動」中，評為優秀論文。
4. 王偉.阿莫西林可溶片的葯動學及生物利用度.中國醫院葯學雜志，2000；20（9）：87-88
5. 王偉.某院1999-2001年抗感染葯物利用分析及對策.中國葯師，2003；6（12）：827-829
6. 王偉，方學明.淺析我院的葯品結構與促進合理用葯措施.中國醫院葯學雜志，2003；23（9）：76-77
7. 王偉.葯物引起蕁麻疹1例.葯物流行病學雜志，2003；12（6）：285
8. 《HMG-COA還原酶抑制劑的葯理作用及臨床應用》經專家評審在「98首屆中國葯師周-世紀之交的中國葯學」大會進行交流。
9. 王偉，方學名.150份葯歷的用葯合理性分析.中國葯師，2002；5（4）：232-241
10. 王偉，沈曉英，曹華，等.地卡因膠漿的制備與應用.醫葯導報，2005；24：110
11. 王偉.高血壓患者的葯學監護.中國葯師2004；7：12-13
『柒』 請幫忙找一篇基因工程相關的外文論文再加上翻譯！
Synthesis of optically pure ethyl (S)-4-chloro-3-hydroxybutanoate
by Escherichia coli transformant cells coexpressing
the carbonyl rectase and glucose dehydrogenase genes
由共表達碳醯還原酶和葡萄糖脫氫酶的大腸桿菌轉化細胞合成
純光學（S）-4-氯-3-羥基丁酸乙酯
Abstract The asymmetric rection of ethyl 4-chloro-3-
oxobutanoate (COBE) to ethyl (S)-4-chloro-3-hydroxybutanoate
((S)-CHBE) was investigated. Escherichia coli cells expressing both the carbonyl rectase (S1) gene from Candida magnoliae and the glucose dehydrogenase (GDH) gene from Bacillus megaterium were used as the
catalyst. In an organic-solvent-water two-phase system,(S)-CHBE formed in the organic phase amounted to 2.58 M (430 g/l), the molar yield being 85%. E. coli transformant cells coprocing S1 and GDH accumulated 1.25 M (208 g/l) (S)-CHBE in an aqueous monophase system by continuously feeding on COBE, which is unstable in an aqueous solution. In this case, the calculated turnover of NADP+ (the oxidized form of nicotinamide adenine dinucleotide phosphate) to CHBE was 21,600 mol/mol. The optical purity of the (S)-CHBE formed was 100% enantiomeric excess in both systems. The aqueous system used for the rection reaction involving E. coli HB101 cells carrying a plasmid containing the S1 and GDH genes as a catalyst is simple. Furthermore, the system does not require the addition of commercially available GDH or an organic solvent. Therefore this system is highly advantageous for the practical synthesis of optically pure (S)-CHBE.
本本篇文獻研究了利用COBE不對稱合成（S）-4-氯-3-羥基丁酸乙酯（CHBE）。大腸桿菌細胞作為催化劑同時表達了來自念珠菌屬magnoliae的碳醯還原酶和來自巨大芽孢桿菌的葡萄糖脫氫酶基因。在水/有機溶劑兩相體系中，(S)-CHBE在有機相中的濃度可以達到2.58M（430g/l），摩爾產率達到85%。大腸桿菌的副產物S1和GDH也達到了1.25M（208g/l），COBE在水相中不穩定，所以(S)-CHBE可以在水單相中不停的生成。在這種情況下，適當的從NADP+到CHBE的轉變達到了21,600 mol/mol。所形成的CHBE的旋光度在這種體系中100%對映體過量。在水相中用攜帶含有S1和GDH基因質粒的E. coli HB101作為催化劑不對稱還原是比較簡單的。並且，這種體系並不額外需要商業GDH或者有機溶劑。因此，這種體系對於實際合成純光學活性的(S)-CHBE是非常方便的。
Optically active 4-chloro-3-hydroxybutanoic acid esters are useful chiral building blocks for the synthesis of pharmaceuticals. The (R)-enantiomer is a precursor of L-carnitine (Zhou et al. 1983), and (S)-enantiomer is an important starting material for hydroxymethylglutaryl- CoA (HMG-CoA) rectase inhibitors (Karanewsky et
al. 1990). Many studies have described the microbial or enzymatic asymmetric rection of 4-chloro-3-oxobutanoic acid esters (Aragozzini and Valenti 1992; Bare et al.1991; Hallinan et al. 1995; Patel et al. 1992; Shimizu et al. 1990; Wong et al. 1985) based on the rection by baker』s yeast (Zhou et al. 1983).We have previously showed that Candida magnoliae AKU4643 cells reced ethyl 4-chloro-3-oxobutanoate (COBE) to (S)-CHBE with an optical purity of 96% enantiomeric excess (e.e.) (Yasohara et al. 1999). As this yeast has at least three different stereoselective rectases (Wada et al. 1998, 1999a, b), the (S)-CHBE proced by this yeast was not optically pure. From among these three enzymes, an NADPH-dependent carbonyl rectase, designated as S1, was purified and characterized in some detail (Wada et al. 1998). We cloned and sequenced the gene encoding S1 and overexpressed it in Escherichia coli cells. This E. coli transformant reced COBE to optically pure (S)-CHBE in the presence of glucose, NADP+, and commercially available glucose dehydrogenase (GDH) as a cofactor generator (Yasohara
et al. 2000).
Here, we describe the construction of three E. coli transformants coexpressing the S1 from C. magnoliae and GDH from Bacillus megaterium genes and analyze the rection of COBE catalyzed by these strains. Previous reports on the enzymatic rection of COBE to (R)-CHBE with an optical purity of 92% e.e. (Kataoka et al. 1999; Shimizu et al. 1990) recommended an organic- solvent two-phase system reaction for an enzymatic or microbial rection, because the substrate (COBE) is unstable in an aqueous solvent and inactivates enzymes. We examined the rection of COBE to optically pure (S)-CHBE by E. coli transformants in a water monophase system reaction and discuss the possible use of this type of reaction system in instrial applications。
具有旋光性的（S）-4-氯-3-羥基丁酸乙酯在葯物制劑的合成中是重要的手性化合物。其右旋體是L-卡尼汀的前體，其左旋體是羥甲基戊二醯輔酶A還原酶抑制劑的起始材料。許多研究描述了以麵包酵母為基礎微生物或者酶的COBE的不對稱還原。我們先前已經知道利用來自念珠菌屬magnoliae AKU4643 細胞催化COBE生成光學純度96%的CHBE。這種酵母至少有三種立體選擇性的還原酶，這種酵母產生的CHBE並非純光學的，在這三種酶之中，NADPH-依賴碳醯還原酶，我們克隆並測序編碼S1的基因，並在大腸桿菌中過表達。大腸桿菌轉化細胞在葡萄糖，NADP+和商業化的葡萄糖脫氫酶作為輔酶因子的啟動子催化COBE生成純光學的CHBE。
我們構建這三種大腸桿菌轉化細胞共表達來自的S1和來自巨大芽孢桿菌的GDH，並分析COBE被這幾種菌株催化還原的反應機理。先前的報道表明，利用酶催化還原COBE生成CHBE光學純度可達92%，也提到了因為底物（COBE）在水相中不穩定，並且酶容易鈍化，所以利用酶或者微生物在有機溶劑/水兩相體系中催化反應。我們研究了在水單相體系中由COBE還原生成純光學的CHBE，還討論了這種反應體系在工業應用中可能的用途。
Materials and methods
Bacterial strain and plasmids
The E. coli strains used in this study were JM109 and HB101.Plasmid pGDA2, in which the GDH gene from B. megaterium is inserted into pKK223-3, was kindly provided by Professor I. Urabe, Osaka University (Makino et al. 1989). Plasmids pSL301 and pTrc99A were purchased from Invitrogen (USA), and Amersham Pharmacia Biotech (UK), respectively. Plasmids pUC19 and pSTV28 (Homma et al. 1995; Takahashi et al. 1995) were purchased from Takara Shuzo (Japan).
材料和方法
菌株和質粒
本次實驗中使用的大腸桿菌是JM109 and HB101。來自B. megaterium的GDH基因插入到Pkk233-3質粒中，而帶有GDH基因片段的pGDA2質粒由到由大阪大學的urabe教授提供。質粒pSL301和 pTrc99A是由美國的Invitrogen公司和英國的公司分別購買的。質粒pUC19和pST28是由日本takara公司購買的。
The recombinant plasmid used in this study was constructed as follows (Fig. 1): Plasmid pGDA2 was double-digested with EcoRI and PstI to isolate a DNA fragment of about 0.9 kilobase pairs (kb) including the GDH gene. This fragment was inserted into the EcoRI-PstI site of plasmid pSL301 to construct plasmid pSLG. Plasmid pSLG was double-digested with EcoRI and XhoI to isolate a DNA fragment of about 0.9 kb including the GDH gene.
這次實驗使用的重組質粒構建如下：質粒pGDA2 被EcoRI 和 PstI雙酶切從而分離出一個大小約為0.9kb的包含有GDH基因的DNA片段。這個片段被插入到質粒Psl301的EcoRI-PstI酶切位點從而構建出質粒pSLG。質粒pSLG被EcoRI和XhoI
To construct plasmid pNTS1G, this 0.9-kb fragment was inserted into the EcoRI-SalI site of pNTS1, which was constructed to overproce S1 as described previously (Yasohara et al. 2000). To construct plasmid pNTGS1, plasmid pNTG was first generated. Two synthetic primers (primer 1, TAGTCCATATGTATAAAGATTTAG,and primer 2 TCTGAGAATTCTTATCCGCGTCCT) were prepared for polymerase chain reaction (PCR) using pGDA2 as the template. The PCR-generated fragment was double- digested with NdeI and EcoRI and then inserted into the NdeI EcoRI site of plasmid pUCNT, which was constructed from pUC19 and pTrc99A, as reported (Nanba et al. 1999), to obtain pNTG. To construct plasmid pNTGS1, two synthetic primers (primer 3, , and primer 4, ) were prepared using pUCHE, which contains the S1 gene as the template. The PCR-generated fragment was double-digested with EcoRI and SalI and then inserted into the EcoRI-SalI site of pNTG to obtain pNTGS1. Plasmid pNTS1G, pNTGS1 or pNTG was transformed into E. coli HB101.
構建pNTS1是為了過表達前文所提到的S1，這個0.9kb大小的片段被插入到pNTS1的EcoRI-SalI酶切位點從而構建pNTS1G。為了構建質粒pNTGS1，首先需要構建pNTG。兩個合成引物（引物1，TAGTCCATATGTATAAAGATTTAG和引物2，TCTGAGAATTCTTATCCGCGTCCT）和作為模板的pGDA2是PCR反應需要的。PCR得到的片段是由NdeI 和EcoRI雙酶切和並插入到質粒pUCNT的NdeI EcoRI酶切位點來得到pNTG。根據報道，pUCNT是由pUC19和 pTrc99A構建而來。為了構建質粒pNTGS1，兩個合成引物(引物 3, , and 引物 4, )，包括了S1基因作為模板。Pcr產物片段被EcoRI和SalI雙酶切然後被插入到pntg的EcoRI-SalI酶切位點得到pntg1.質粒pNTS1G, pNTGS1或者 pNTG都是導入大腸桿菌HB101.
Plasmid pGDA2 was double-digested with EcoRI and PstI to isolate a DNA fragment of about 0.9 kb including the GDH gene. To construct plasmid pSTVG, this fragment was inserted into the EcoRI-PstI site of plasmid pSTV28. Plasmid pSTVG was transformed into E. coli HB101.
質粒pGDA2被EcoRI 和 PstI雙酶切得到包含GDH基因的0.9kb大小的DNA片段。為了構建pSTVG質粒，這個片段被插入到pSTV28質粒的EcoRI-PstI的酶切位點。pSTVG質粒被導入到E. coli HB101。
Medium and cultivation
The 2×YT medium comprised 1.6% Bacto-tryptone, 1.0% yeast
extract, and 0.5% NaCl, pH 7.0. E. coli HB 101 carrying pNTS1,
pNTG, pNTS1G, or pNTGS1 was inoculated into a test tube containing
2 ml 2×YT medium supplemented with 0.1 mg/ml ampicillin,
followed by incubation at 37 °C for 15 h with reciprocal shaking.
This preculture (0.5 ml) was transferred to a 500-ml shaking
flask containing 100 ml 2×YT medium. The cells were cultivated
at 37 °C for 13 h with reciprocal shaking. E. coli HB101 carrying
pNTS1 and pSTVG was similarly cultivated in 2×YT medium
supplemented with 0.1 mg/ml ampicillin and 0.1 mg/ml chloramphenicol.
培養基和培菌
2*YT培養基 包含有1.6%細菌用胰蛋白腖，1.0%酵母提取物，0.5% NaCl，pH7.0.
攜帶有pNTS1,pNTG, pNTS1G, 或 pNTGS1的大腸桿菌HB101被接種到有0.1mg/ml氨苄青黴素的2ml的2*YT培養基，37°C搖床15小時。將0.5ml菌液接種到100ml2*YT培養基的500ml燒瓶中。在37°C搖床培養13小時。攜帶有pNTS1 和 pSTVG質粒的大腸桿菌HB101在2*YT培養基中培養方法相似，只是培養基中要加入0.1 mg/ml的氨苄青黴素和 0.1 mg/ml的氯黴素。
Preparation of cell-free extracts and the enzyme assay
Cells were harvested from 100 ml of culture broth by centrifugation, suspended in 50 ml of 100 mM potassium phosphate buffer (pH 6.5), and then disrupted by ultrasonication. The cell debris was removed by centrifugation; the supernatant was recovered as the cell-free extract. Carbonyl rectase S1 activity (COBE-recing activity) was determined spectrophotometically as follows: The assay mixture consisted of 100 mM potassium phosphate buffer (pH 6.5), 0.1 mM NADPH, and 1 mM COBE. The reactions were incubated at 30 °C and monitored for the decrease in absorbance at 340 nm. The assay mixture for GDH activity consisted of 1 M Tris-HCl buffer (pH 8.0), 100 mM glucose, and 2 mM NADP+. The reactions were incubated at 25 °C and monitored for the increase in absorbance at 340 nm. One unit of S1 or GDH was defined as the amount catalyzing the rection of 1 μmol NADP+ or oxidation of 1 μmol NADPH per minute, respectively. Protein concentrations were measured with a protein
assay kit containing Coomassie brilliant blue (Nacalai Tesque, Japan),
using bovine serum albumin as the standard (Bradford 1976).
無細胞抽提液和酶鑒定
將100ml培養液離心收獲菌體，用50ml0.1mol/LpH為6.5的磷酸緩沖液懸浮，然後超聲粉碎。細胞碎片通過離心可以去除，收集上層清液就是無細胞抽提物。碳醯還原酶S1的活性由分光光度計測量如下：測定的混合物包括：0.1mol/LpH6.5的磷酸二氫鉀緩沖液，0.1mMNADPH和1mMCOBE。反應在30°C條件下反應，並且隨時監測其在340nm處的吸光值。測GDH混合物包括：1M pH 8.0的Tris-HCl的緩沖液，100mM的葡萄糖，2mM的NADP+。反應在25°C下進行，監測其在340nm處的吸光值。一個單位S1或GDH被定義為每分鍾催化還原1μmol NADP+或氧化1 μmol NADPH的量。蛋白質的測定通過含有考馬斯亮藍的蛋白質測定試劑利用牛血清白蛋白作為標准進行測定。
Study of enzyme stability
One milliliter of 100 mM potassium phosphate buffer (pH 6.5) containing the cell-free extracts of E. coli HB101 carrying pNTS1 (S1: 20 U/ml) was mixed with an equal volume of each test organic solvent in a closed vessel. After the mixture was shaken at 30 °C for 48 h, the remaining enzyme activities in an aqueous phase were assayed as described above. The mixture, containing 100 mM potassium phosphate buffer (pH 6.5), S1 (20 U/ml), and various concentrations of CHBE, was incubated at 30 °C for 24 h in order to study the enzyme』s stability in the presence of CHBE.The remaining enzyme activities were assayed as described above.
酶穩定性的研究
一毫升含有含有pNTS1質粒的E. coli HB101的無細胞抽提液的100mM磷酸氫二鉀緩沖液（pH6.5）與等體積的有機溶劑混合。混合物在30 °C震搖48小時後，水相中殘留的酶活力即是上述的酶活力。
COBE rection with E. coli cells expressing the S1 gene and E. coli cells expressing GDH genes in a two-phase system reaction
The reaction mixture comprised 15 ml culture broth of E. coli HB101 carrying pNTG, 17 ml culture broth of E. coli HB101 carrying pNTS1, 1.6 mg NADP+, 4 g glucose, 2.5 g COBE, 25 ml n-butyl acetate, and about 25 mg Triton X-100. The pH of the reaction mixture was controlled at 6.5 with 5 M sodium hydroxide. At 2 h, 1.25 g COBE and 2.5 g glucose were added to the reaction mixture. To compare the reaction by E. coli transformant coexpressing the GDH and S1 genes, 30 ml culture broth of E. coli
HB101 carrying pNTS1G was used instead of culture broth of E. coli HB101 carrying pNTG and E. coli HB101 carrying pNTS1. Other components and the procere were the same as described above.
表達S1基因和GDH基因的大腸桿菌細胞在兩相反應體系中的還原反應
混合物包含有帶有pNTG質粒的大腸桿菌HB101的菌液15ml，pNTS1質粒的大腸桿菌HB101的菌液17ml，1.6 mg NADP+，4 g葡萄糖，2.5g的COBE，25ml的n-butyl acetate丁醯醋酸鹽和大約25mg的聚乙二醇辛基苯基醚Triton X-100。用5M的NaOH溶液將pH控制在6.5。在反應兩小時後，加入1.25gCOBE和2.5g葡萄糖到該混合物中。比較大腸桿菌轉化細胞共表達GDH和S1基因，攜帶有pNTS1G質粒的大腸桿菌HB10130ml菌液取代了攜帶有pNTG和pNTS1質粒的大腸桿菌HB101菌液。其他的成分和步驟和上述的方法相似。
COBE rection to (S)-CHBE in a two-phase system reaction
The reaction mixture contained 50 ml of culture broth of an E. coli HB101 transformant, 3.2 mg NADP+, 11 g glucose, 10 g COBE, 50 ml n-butyl acetate, and about 50 mg Triton X-100. The reaction mixture was stirred at 30 °C, and the pH was controlled at 6.5 with 5 M sodium hydroxide. Five grams of COBE/5.5 g glucose and 10 g COBE/11 g glucose were added to the reaction mixture at 3 h and 7 h, respectively; 3.2 mg NADP+ was added at 26 h.
COBE在兩相系統中還原生成（S）-CHBE
反應混合物包含50ml E. coli HB101轉化細胞的培養液，3.2mgNADP+,11g
葡萄糖，10gCOBE，50ml丁醯醋酸，和大概50mg聚乙二醇辛基苯基醚Triton X-100.
在30°C溫度下將其混合均勻，並用5M的NaOH溶液將pH控制在6.5。在第3小時加入5gCOBE和5.5g葡萄糖或者在第7小時加入10gCOBE和11g葡萄糖，分別在第26小時加入3.2gNADP+。
COBE rection to (S)-CHBE in an aqueous system reaction
The reaction mixture was made up of 50 ml of culture broth of an E. coli HB101 transformant, 3.1 mg NADP+, 11 g glucose, and about 50 mg Triton X-100. The reaction mixture was stirred at 30 °C. Fifteen grams of COBE was fed continuously by means of a micro-feeding machine at a rate of about 0.02 g/min for about 12 h. The pH of the reaction mixture was controlled at 6.5 with 5 M sodium hydroxide. The reaction mixture was extracted with 100 ml ethyl acetate. The organic layer was dried over anhydrous sodium sulfate and then evaporated in vacuo.
COBE在水相中還原成(S)-CHBE的反應
反應的體系是由50ml大腸桿菌HB101轉化細胞的菌液，3.1mgNADP+，11g葡萄糖和大約50mg聚乙二醇辛基苯基醚Triton X-100。反應混合物在30°C15mg的COBE通過微量添加機器以0.02 g/min的速率連續12小時恆定的加入到體系中。用5M的NaOH溶液將pH控制在6.5。反應混合物用100ml乙酸乙酯萃取。有機層用無水硫酸鈉吸干，並在真空中脫水。
Analysis
The organic layer was obtained on centrifugation of the reaction mixture and was assayed for CHBE and COBE by gas chromatography. Optical purity of CHBE was analyzed by high-performance liquid chromatography (HPLC), as described previously (Yasohara et al. 1999).
Enzymes and chemicals
Restriction enzymes and DNA polymerase were purchased from
Takara Shuzo (Japan). COBE (molecular weight: 164.59) was purchased
from Tokyo Kasei Kogyo (Japan). Racemic CHBE (molecular
weight: 166.60) was synthesized by rection of COBE with
NaBH4. All other chemicals used were of analytical grade and
commercially available.
分析
離心反應混合物得到的有機層通過氣相色譜法測定其CHBE和COBE。COBE的光學純度如前所述通過高效液相色譜法進行分析。
酶和化學試劑
限制性內切酶和DNA聚合酶由takara公司購得，COBE（分子量：164.59）由東京Tokyo Kasei Kogyo公司購得，消旋體CHBE（分子量166.6）通過COBE及NaBH4合成。所有其他化學試劑都是分析等級和商業化的試劑。
Construction of E. coli transformants overprocing S1 and GDH
To express the carbonyl rectase S1 and GDH genes in the same E. coli cells, four expression vectors were constructed (Fig. 1). Plasmids pNTS1G and pNTGS1 contain the S1 gene from C. magnoliae, the GDH gene from B. megaterium, the lac promoter derived from pUC19, and the terminator derived from pTrc99A. Plasmid pNTS1 contains the S1 gene, the lac promoter derived from pUC19, and the terminator derived from pTrc99A. The enzyme activities in cell-free extracts of the E. coli transformants are shown in Table 1. E. coli HB101 cells carrying the vector plasmid pUCNT had no detectable S1 or GDH activity. E. coli HB101 carrying either pNTS1G or pNTGS1 showed S1 and GDH activity without isopropyl-β-D-thiogalactopyranoside (IPTG) inction. The S1 activities of these two transformants were lower than the GDH activities. To obtain a transformant whose S1 activity was equal to or greater than the level of GDH activity, we used a lower vector, pSTV28 (Homma et al. 1995; Takahashi et al. 1995), to express the GDH gene. It may be possible to raise the S1 activity by lowering the GDH activity. Plasmid pSTVG contains the GDH gene, the lac promoter, the chloramphenicol resistance gene, and the replicative origin derived from pACYC184 for compatibility with the plasmid pNTS1. In E. coli HB101 carrying pNTS1 and pSTVG, the S1 activity was higher than the GDH activity, but this GDH
level may be too low to regenerate in a COBE rection reaction as described below.
過產生S1和GDH的大腸桿菌轉化細胞的構建
為了在同一大腸桿菌細胞中表達碳醯還原酶S1和GDH基因，要構建四個表達型載體。質粒pNTS1G 和 pNTGS1包含有來自C. magnoliae的S1基因，來自B. megaterium的GDH基因，來自pUC19的LAC啟動子，從pTrc99A的來的終止子，質粒pNTS1包含有S1基因，來自pUC19的LAC啟動子，從pTrc99A的來的終止子。在大腸桿菌轉化細胞的無細胞抽提物的酶活力如表一所示。攜帶有運輸質粒pUCNT的大腸桿菌細胞無法檢測到其S1和GDH活性。攜帶有pNTS1G 或 pNTGS1質粒在沒有IPTG的誘導下有S1和GDH的活性。在這兩個轉化菌種中，S1的活力小於GDH的活力。為了得到S1活性等於或者大於GDH的大腸桿菌轉化菌株，我們使用低拷貝的載體pSTV28，來表達GDH基因。它可能可以通過降低GDH的活性從而提高S1的活性。質粒pSTVG包含有GDH基因，lac啟動子，和氯黴素抗性基因，以及與pNTS1具有相容性的從pACYC184得來的復制起始位點。在攜帶有pNTS1和pSTVG的大腸桿菌轉化細胞中，S1的活性要高於GDH的活性，但是GDH的活性可能會太低而在COBE還原反應中不能再生。
太長了，字數有限制，所以不能發完。分數我無所謂啦，我很少登錄的。這應該算是基因工程的吧，是我以前自己翻的，不是很好。如果你要的話可以聯系我的郵箱。[email protected]
『捌』 黃路生的主要論著
發表論文160多篇，其中在20種國際性學術期刊發表SCI論文57篇；以通訊作者在17種國際性學術期刊發表SCI論文46篇，其中在本學科領域國際學術期刊排名前15% （Top 15%）的刊物發表論文21篇，有14篇發表於排名第1的刊物《Animal Genetics》，出版專（譯）著教材2部，參編全國農林院校統編教材《動物遺傳學》1部 。 權威核心刊物論文
1、Three novel quantitative trait loci for skin thickness in swine identified by linkage and genome-wide association studies；Huashui Ai, Shijun Xiao, Zhiyan Zhang, Bin Yang, Lin Li, Yuanmei Guo, Guoshan Lin, Jun Ren andLusheng Huang；Animal GeneticsVolume 45, Issue 4, pages 524–533, August 2014
2、Genetic Diversity, Linkage Disequilibrium and SelectionSignatures in Chinese and Western Pigs Revealed byGenome-Wide SNP Markers；Huashui Ai, Lusheng Huang, Jun Ren；PLoS ONE February 7, 2013
3、Fine mapping of a QTL for ear size on porcinechromosome 5 and identification of high mobilitygroup AT-hook 2 (HMGA2) as a positionalcandidate gene；Pinghua Li, Shijun Xiao, Na Wei, Zhiyan Zhang, Ruihua Huang, Yueqing Gu, Yuanmei Guo, Jun Ren,Lusheng Huang and Congying Chen；Genetics Selection Evolution 2012, 44:6
4、A comprehensive survey of number variation in 18 diverse pig populations and identification of candidate number variable genes associated with complex traits；Congying Chen, Lusheng Huang etc.；BMC Genomics 2012; 13: 733.
5、A global view of porcine transcriptome in threetissues from a full-sib pair with extremephenotypes in growth and fat deposition by paired-end RNA sequencing；Congying Chen,Huashui Ai, Jun Ren, Wanbo Li,Pinghua Li,Ruimin Qiao,Jing Ouyang, Ming Yang, Junwu Maand Lusheng Huang；BMC Genomics 2011, 12:448
6、The porcine MUC20 gene: molecular characterization and its association with susceptibility to enterotoxigenic Escherichia coli F4ab/ac；Ji H, Ren J, Yan X, Huang X, Zhang B, Zhang Z, Huang L.；Mol Biol Rep2011 Mar;38(3):1593-601
7、Adhesion phenotypes of pigs of Chinese and Western breeds and a White Duroc-Erhualian crossbreed with regard to susceptibility to enterotoxigenic Escherichia coli with fimbrial adhesins K99, 987P, and F41；Xueming Yan, Xiang Huang, Jun Ren, Jing Ouyang, MS Ming Yang, MS Pengfei Han, MS Lusheng Huang；American Journal of Veterinary ResearchJanuary 2011, Vol. 72, No. 1, Pages 80-84
8、Genetic variation within coat color genes of MC1R and ASIP in Chinese brownish red Tibetan pigs；MAO Huirong,REN Jun, DING Nengshui, XIAO Shijun, HUANG Lusheng；Animal Science JournalDecember 2010,630-634
9、A whole genome scanning for quantitative trait loci on traits related to sperm quality and ejaculation in pigs；Yuyun Xing, Jun Ren, Dongren Ren, Yuanmei Guo, Yanbo Wu, Guangcheng Yang, Huirong Mao, Bertram Brenig, Lusheng Huang；Animal Reproction ScienceAugust 2009, Pages 210–218
10、A genome-wide scan for quantitative trait loci affecting limb bone lengths and areal bone mineral density of the distal femur in a White Duroc × Erhualian F2 population；Huirong Mao, Yuanmei Guo, Guangcheng Yang, Bin Yang, Jun Ren, Sanfeng Liu, Huashui Ai, Junwu Ma, Bertram Brenig and Lusheng Huang；BMC Genetics 2008, 9:63
11、Quantitative trait loci for porcine baseline erythroid traits at three growth ages in a White Duroc � Erhualian F2 resource population；Zheng Zou; Jun Ren; Xueming Yan; Xiang Huang; Shujin Yang; Zhiyan Zhang; Bin Yang; Wanbo Li; Lusheng Huang；Mammalian Genome Sep2008, Vol. 19 Issue 9, p640
12、Isolation and molecular characterization of the porcine stearoyl-CoA desaturase gene；Ren J, Knorr C, Huang L, Brenig B；Gene2004 Sep 29;340(1):19-30.
13、Genetic variations of the porcine PRKAG3 gene in Chinese indigenous pig breeds；Lu-Sheng Huang,Jun-Wu Ma；Genetics Selection Evolution 2004, 36:481-486
重要核心刊物論文 作者題名來源黃路生找准問題 提升我國種豬育種水平《中國豬業》 2013年10期 黃路生種豬選育技術的現狀與趨勢《中國豬業》 2012年04期 任軍 黃路生等仔豬斷奶前腹瀉抗病基因育種技術的創建及應用《豬業科學》2012年01期 肖石軍 黃路生等採用全基因組掃描法定位影響豬後腿質量QTL《畜牧獸醫學報》2009年10期 劉三鳳 黃路生等雞SCD和Sirt1基因的SNP搜尋及其遺傳多樣性分析《畜牧獸醫學報》 2008年03期 晏學明 黃路生等不同品種豬α1-岩藻糖轉移酶基因遺傳變異初析《中國畜牧雜志》 2004年03期 鄧素華 黃路生等豬黑素皮質素受體1（MC1R）基因與毛色表型的研究《遺傳學報》2003年10期 陳從英 黃路生等牛早期胚胎性別鑒定PCR反應體系的優化研究《畜牧獸醫學報》 2003年03期 任軍 黃路生 等24個中外豬種（群）的AFLP多態性及其群體遺傳關系《遺傳學報》2002年09期 陳克飛 黃路生豬雌激素受體（ESR）基因對產仔數性狀的影響《遺傳學報》 2000年10期 黃路生 羅明等中俄豬16個遺傳標記基因位點的多態與繁殖性狀的相關研究《畜牧獸醫學報》1999年05期 丁能水 黃路生等氟烷基因對豬繁殖性能影響的研究《江西農業大學學報》1999年04期 林樹茂 黃路生等中國地方豬種TfC的基因頻率與繁殖性能相關性分析《江西畜牧獸醫雜志》1996年02期 黃路生遺傳標記——現代豬育種工作的一項生物新技術《全球科技經濟瞭望》1993年03期 書名出版社時間類型動物遺傳學中國農業出版社2003參編 江西農業大學校史（二）江西高校出版社2010合著
『玖』 宋保亮的發表文章
Representative Publications:
1) Chu BB, Liao YC, Qi W, Xie C, Du X, Wang J, Yang H, Miao HH, Li BL and Song BL*. Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts. Cell, 161(2):291-306, 2015
2) Li PS, Fu ZY, Zhang YY, Xu CQ, Ma YT, Li BL and Song BL*. The clathrin adaptor Numb regulates intestinal cholesterol absorption through dynamic interaction with NPC1L1. Nature Medicine, 20(1): 80-86, 2014
3) Liu TF, Tang JJ, Li PS, Shen Y, Li JG, Miao HH, Li BL* and Song BL*. Ablation of gp78 in liver improves hyperlipidemia and insulin resistance by inhibiting SREBP to decrease lipid biosynthesis. Cell Metabolism, 16: 213-225, 2012
4) Tang JJ, Li JG, Qi W, Qiu WW, Li PS, Li BL and Song BL*. Inhibition of SREBP by a small molecule, betulin, improves hyperlipidemia and insulin resistance and reces atherosclerotic plaques. Cell Metabolism, 13: 44-56, 2011
5) Ge L, Wang J, Qi W, Miao HH, Cao J, Qu YX, Li BL and Song BL*. The cholesterol absorption inhibitor ezetimibe acts by blocking the sterol-inced internalization of NPC1L1. Cell Metabolism,7: 508-519, 2008
6) Cao J, Wang J, Qi W, Miao HH, DeBose-Boyd RA, Wang J, Li BL* and Song BL*. Ufd1 is a cofactor of gp78 and plays a key role in cholesterol metabolism. Cell Metabolism, 6:115-128, 2007
7) Ge L, Qi W, Wang LJ, Miao HH, Qu YX, Li BL and Song BL*. Flotillins play an essential role in Niemann-Pick C1 Like 1-mediated cholesterol uptake. PNAS, 108(2): 551-6, 2011
8) Song BL, Sever N, and DeBose-Boyd RA. Gp78, a membrane anchored ubiquitin ligase, associates with Insig-1 and couples sterol-regulated ubiquitination to degradation of HMG CoA rectase. Molecular Cell. 19(6):829-840, 2005
9) Song BL, Javitt NB, and DeBose-Boyd RA. Insig-mediated degradation of HMG CoA rectase stimulated by lanosterol, an intermediate in the synthesis of cholesterol. Cell Metabolism, 1: 179-189, 2005
10) Sever N#, Song BL#, Yabe D#, Goldstein JL, Brown MS, and DeBose-Boyd RA. Insig-dependent ubiquitination and degradation of mammalian 3-Hydroxy-3-methylglutaryl-CoA rectase stimulated by sterols and geranylgeraniol. J Biol Chem, 278: 52479-52490, 2003
Other Publications:
11) Wei J, Fu ZY, Li PS, Miao HH, Li BL, Ma YT, Song BL*. The Clathrin Adaptor Proteins ARH, Dab2, and Numb Play Distinct Roles in Niemann-Pick C1-Like 1 Versus Low Density Lipoprotein Receptor-mediated Cholesterol Uptake. J Biol Chem, 289(48):33689-700, 2014
12) Jiang W, Tang JJ, Miao HH, Qu YX, Qin J, Xu J, Yang J, Li BL, Song BL*. Forward Genetic Screening for Regulators Involved in Cholesterol Synthesis Using Validation-Based Insertional Mutagenesis. PLoS One, 9(11):e112632, 2014
13) Jiang W, Song BL*. Ubiquitin ligases in cholesterol metabolism. Diabetes Metab J. 38(3):171-80, 2014
14) Rogers MA, Liu J, Song BL, Li BL, Chang CC, Chang TY. Acyl-CoA: cholesterol acyltransferases (ACATs/SOATs): Enzymes with multiple sterols as substrates and as activators. J Steroid Biochem Mol Biol. S0960-0760(14): 00207-6, 2014
15) Xiao X*, Song BL. SREBP: a novel therapeutic target.Acta Biochim Biophys Sin (Shanghai).45(1):2-10, 2013
16) Song BL*. A special issue on 'Metabolism'. Acta Biochim Biophys Sin (Shanghai).45(1):1, 2013
17) Xu J, Hu G, Lu M, Xiong Y, Li Q, Chang CC, Song BL, Chang TY, Li BL. MiR-9 reces human acyl-coenzyme A:cholesterol acyltransferase-1 to decrease THP-1 macrophage-derived foam cell formation.Acta Biochim Biophys Sin (Shanghai).45(11):953-62, 2013
18) Lu M, Hu XH, Li Q, Xiong Y, Hu GJ, Xu JJ, Zhao XN, Wei XX, Chang CC, Liu YK, Nan FJ, Li J, Chang TY, Song BL*, Li BL*.A specific cholesterol metabolic pathway is established in a subset of HCCs for tumor growth.J Mol Cell Biol. 5:404-15,2013
19) Hu GJ, Chen J, Zhao XN, Xu JJ, Guo DQ, Lu M, Zhu M, Xiong Y, Li Q, Chang CC, Song BL, Chang TY, Li BL. Proction of ACAT1 56-kDa isoform in human cells via trans-splicing involving the ampicillin resistance gene.Cell Res. 23(8):1007-24, 2013
20) Liu Y, Xu XH, Chen Q, Wang T, Deng CY, Song BL, Du JL, Luo ZG. Myosin Vb controls biogenesis of post-Golgi Rab10 carriers ring axon development.Nat Commun. 4:2005.2013
21) Xie C, Zhou ZS, Li N, Bian Y, Wang YJ, Wang LJ, Li BL* and Song BL*. Ezetimibe blocks the internalization of NPC1L1 and cholesterol in mouse small intestine. J Lipid Res, 53: 2092-2101, 2012
22) Wang LJ and Song BL*. Niemann-Pick C1-Like 1 and cholesterol uptake. Biochim Biophys Acta, 1821(7):964-72, 2012
23) Xie C, Li N, Chen ZJ, Li BL, Song BL*. The small GTPase Cdc42 interacts with Niemann-Pick C1 Like 1 (NPC1L1) and controls its movement from endocytic recycling compartment to plasma membrane in a cholesterol dependent manner.J Biol Chem, 286(41):35933-42, 2011
24) Zhang JH, Ge L, Qi W, Zhang L, Miao HH, Li BL, Yang M and Song BL*. The N-terminal domain of NPC1L1 protein binds cholesterol and plays essential roles in cholesterol uptake. J Biol Chem, 286(28): 25088-97, 2011
25) Wang LJ, Wang J, Li N, Ge L, Li BL and Song BL*. Molecular characterization of the NPC1L1 variants identified from cholesterol low absorbers. J Biol Chem, 286(9): 7397-7408, 2011
26) Miao HH, Jiang W, Ge L, Li BL, Song BL*. Tetra-glutamic acid resies adjacent to Lys248 in HMG-CoA rectase are critical for the ubiquitination mediated by gp78 and UBE2G2. Acta Biochim Biophys Sin, 42(5): 303-310, 2010
27) Chu BB, Ge L, Xie C, Zhao Y, Miao HH, Wang J, Li BL and Song BL*. Requirement of Myosin Vb/Rab11a/Rab11-FIP2 complex in cholesterol-regulatedtranslocation of Niemann-Pick C1 Like 1 protein to the cell surface. J Biol Chem, 284: 22481-90, 2009.
28) Wang J, Chu BB, Ge L, Li BL, Yan Y* and Song BL*. Membrane topology of human NPC1L1, a key protein in enterohepatic cholesterol absorption. J Lipid Res, 50: -62, 2009
29) Lei L, Xiong Y, Chen J, Yang JB, Wang Y, Yang XY, Chang CCY, Song BL, Chang TY and Li BL*. TNF-alpha stimulates the ACAT1 expressionin differentiating monocytes to promote the CE-laden cell formation. J Lipid Res, 50: 1057-67, 2009
30) Zhao XN, Chen J, Lei L, Hu GJ, Xiong Y, Xu JJ, Li Q, Yang XY, Chang C, Song BL, Chang TY and Li BL. The optional long 5'-untranslated region of human ACAT1 mRNAs impairs the proction of ACAT1 protein by promoting its mRNA decay. Acta Biochim Biophys Sin, 41: 30-41, 2009
31) Chen J, Zhao XN, Yang L, Hu GJ, Lu M, Xiong Y, Yang XY, Chang CC, Song BL, Chang TY, Li BL. RNA secondary structures located in the interchromosomal region of human ACAT1 chimeric mRNA are required to proce the 56-kDa isoform. Cell Res, 18: 921-936, 2008
32) Qi W and Song BL*. Dissecting the NPC1L1-mediated cholesterol absorption. Future Lipidology, 3: 481-484, 2008
33) Cao J, Qi W and Song BL*. Tocotrienols and the regulation of cholesterol biosynthesis. Chapter 18 (p237-256) In:Tocotrienols: Beyond Vitamin E,CRC press, 2008
34) Lee JN, Song BL, DeBose-Boyd RA, Ye J. Sterol-regulated degradation of Insig-1 mediated by the membrane-bound ubiquitin ligase gp78. J Biol Chem, 281:39308-39315, 2006
35) Song BL* and Debose-Boyd RA*. Insig-dependent ubiquitination and degradation of 3-Hydroxy-3-methylglutaryl-Coenzyme A stimulated by {delta}- and {gamma}-Tocotrienols. J Biol Chem, 281: 25054-25061, 2006
36) Song BL, Wang CH, Yao XM, Yang L, Zhang WJ, Wang ZZ, Zhao XN, Yang JB, Qi W, Yang XY, Inoue K, Lin ZX, Zhang HZ, Kodama T, Chang CC, Liu YK, Chang TY and Li BL. Human acyl-CoA:cholesterolacyltransferase 2 gene expression in intestinal Caco-2 cells and in hepatocellular carcinoma. Bio chem J, 394: 617-626, 2006
37) Yao XM, Wang CH, Song BL, Yang XY, Wang ZZ, Qi W, Lin ZX, Chang CC, Chang TY andLi BL. Two human ACAT2 mRNA variants proced by alternative splicing and coding for novel isoenzymes. Acta Biochim Biophys Sin, 37: 797-806, 2005
38) Sever N, Lee PCW, Song BL, Rawson RB, and DeBose-Boyd RA. Isolation of mutant cells lacking Insig-1 through selection with SR-12813, an agent that stimulates degradation of 3-Hydroxy-3-methylglutaryl-Coenzyme A rectase. J Biol Chem, 279: 43136-43147, 2004
39) Song BL and Debose-Boyd RA. Ubiquitination of 3-Hydroxy-3-methylglutaryl-CoA rectase in permeabilized cells mediated by cytosolic E1 and a putative membrane-bound ubiquitin ligase. J Biol Chem, 279: 28798-28806, 2004